Nuclear receptor profile in calvarial bone cells undergoing osteogenic versus adipogenic differentiation

Flavia Q. Pirih, Rosette Abayahoudian, David Elashoff, Farhad Parhami, Jeanne Nervina, Sotirios Tetradis

Research output: Contribution to journalArticle

Abstract

Nuclear receptors (NRs) are key regulators of cell function and differentiation. We examined NR expression during osteogenic versus adipogenic differentiation of primary mouse calvarial osteoblasts (MOBs). MOBs were cultured for 21 days in osteogenic or adipogenic differentiation media. von Kossa and Oil Red O staining, and qRT-PCR of marker genes and 49 NRs were performed. PCR amplicons were subcloned to establish correct sequences and absolute standard curves. Forty-three NRs were detected at days 0-21. Uncentered average linkage hierarchical clustering identified four expression clusters: NRs (1) upregulated during osteogenic, but not adipogenic, differentiation, (2) upregulated in both conditions, with greater upregulation during adipogenic differentiation, (3) upregulated equally in both conditions, (4) downregulated during adipogenic, but not osteogenic, differentiation. One-way ANOVA with contrast revealed 20 NRs upregulated during osteogenic differentiation and 12 NRs upregulated during adipogenic differentiation. Two-way ANOVA demonstrated that 18 NRs were higher in osteogenic media, while 9 NRs were higher in adipogenic media. The time effect revealed 16 upregulated NRs. The interaction of condition with time revealed 6 NRs with higher expression rate during adipogenic differentiation and 3 NRs with higher expression rate during osteogenic differentiation. Relative NR abundance at days 0 and 21 were ranked. Basal ranking changed at least 5 positions for 13 NRs in osteogenic media and 9 NRs in adipogenic media. Osteogenic and adipogenic differentiation significantly altered NR expression in MOBs. These differences offer a fingerprint of cellular commitment and may provide clues to the underlying mechanisms of osteogenic versus adipogenic differentiation.

Original languageEnglish (US)
Pages (from-to)1316-1326
Number of pages11
JournalJournal of Cellular Biochemistry
Volume105
Issue number5
DOIs
StatePublished - Dec 1 2008

Fingerprint

Cytoplasmic and Nuclear Receptors
Bone
Bone and Bones
Osteoblasts
Analysis of variance (ANOVA)
Analysis of Variance
Polymerase Chain Reaction
Dermatoglyphics
Cluster Analysis
Cell Differentiation

Keywords

  • Adipogenic
  • Differentiation
  • Nuclear receptors
  • Osteogenic

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Pirih, F. Q., Abayahoudian, R., Elashoff, D., Parhami, F., Nervina, J., & Tetradis, S. (2008). Nuclear receptor profile in calvarial bone cells undergoing osteogenic versus adipogenic differentiation. Journal of Cellular Biochemistry, 105(5), 1316-1326. https://doi.org/10.1002/jcb.21931

Nuclear receptor profile in calvarial bone cells undergoing osteogenic versus adipogenic differentiation. / Pirih, Flavia Q.; Abayahoudian, Rosette; Elashoff, David; Parhami, Farhad; Nervina, Jeanne; Tetradis, Sotirios.

In: Journal of Cellular Biochemistry, Vol. 105, No. 5, 01.12.2008, p. 1316-1326.

Research output: Contribution to journalArticle

Pirih, FQ, Abayahoudian, R, Elashoff, D, Parhami, F, Nervina, J & Tetradis, S 2008, 'Nuclear receptor profile in calvarial bone cells undergoing osteogenic versus adipogenic differentiation', Journal of Cellular Biochemistry, vol. 105, no. 5, pp. 1316-1326. https://doi.org/10.1002/jcb.21931
Pirih, Flavia Q. ; Abayahoudian, Rosette ; Elashoff, David ; Parhami, Farhad ; Nervina, Jeanne ; Tetradis, Sotirios. / Nuclear receptor profile in calvarial bone cells undergoing osteogenic versus adipogenic differentiation. In: Journal of Cellular Biochemistry. 2008 ; Vol. 105, No. 5. pp. 1316-1326.
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