Nuclear Myosin 1c Facilitates the Chromatin Modifications Required to Activate rRNA Gene Transcription and Cell Cycle Progression

Aishe Sarshad, Fatemeh Sadeghifar, Emilie Louvet, Raffaele Mori, Stefanie Böhm, Bader Al-Muzzaini, Anna Vintermist, Nathalie Fomproix, Ann Kristin Östlund, Piergiorgio Percipalle

Research output: Contribution to journalArticle

Abstract

Actin and nuclear myosin 1c (NM1) cooperate in RNA polymerase I (pol I) transcription. NM1 is also part of a multiprotein assembly, B-WICH, which is involved in transcription. This assembly contains the chromatin remodeling complex WICH with its subunits WSTF and SNF2h. We report here that NM1 binds SNF2h with enhanced affinity upon impairment of the actin-binding function. ChIP analysis revealed that NM1, SNF2h, and actin gene occupancies are cell cycle-dependent and require intact motor function. At the onset of cell division, when transcription is temporarily blocked, B-WICH is disassembled due to WSTF phosphorylation, to be reassembled on the active gene at exit from mitosis. NM1 gene knockdown and motor function inhibition, or stable expression of NM1 mutants that do not interact with actin or chromatin, overall repressed rRNA synthesis by stalling pol I at the gene promoter, led to chromatin alterations by changing the state of H3K9 acetylation at gene promoter, and delayed cell cycle progression. These results suggest a unique structural role for NM1 in which the interaction with SNF2h stabilizes B-WICH at the gene promoter and facilitates recruitment of the HAT PCAF. This leads to a permissive chromatin structure required for transcription activation.

Original languageEnglish (US)
Article numbere1003397
JournalPLoS Genetics
Volume9
Issue number3
DOIs
StatePublished - Apr 15 2013

Fingerprint

Myosins
myosin
rRNA Genes
Chromatin
chromatin
cell cycle
Cell Cycle
transcription (genetics)
ribosomal RNA
gene
actin
Actins
genes
promoter regions
Genes
RNA Polymerase I
Gene Knockdown Techniques
cdc Genes
Chromatin Assembly and Disassembly
acetylation

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Nuclear Myosin 1c Facilitates the Chromatin Modifications Required to Activate rRNA Gene Transcription and Cell Cycle Progression. / Sarshad, Aishe; Sadeghifar, Fatemeh; Louvet, Emilie; Mori, Raffaele; Böhm, Stefanie; Al-Muzzaini, Bader; Vintermist, Anna; Fomproix, Nathalie; Östlund, Ann Kristin; Percipalle, Piergiorgio.

In: PLoS Genetics, Vol. 9, No. 3, e1003397, 15.04.2013.

Research output: Contribution to journalArticle

Sarshad, A, Sadeghifar, F, Louvet, E, Mori, R, Böhm, S, Al-Muzzaini, B, Vintermist, A, Fomproix, N, Östlund, AK & Percipalle, P 2013, 'Nuclear Myosin 1c Facilitates the Chromatin Modifications Required to Activate rRNA Gene Transcription and Cell Cycle Progression', PLoS Genetics, vol. 9, no. 3, e1003397. https://doi.org/10.1371/journal.pgen.1003397
Sarshad, Aishe ; Sadeghifar, Fatemeh ; Louvet, Emilie ; Mori, Raffaele ; Böhm, Stefanie ; Al-Muzzaini, Bader ; Vintermist, Anna ; Fomproix, Nathalie ; Östlund, Ann Kristin ; Percipalle, Piergiorgio. / Nuclear Myosin 1c Facilitates the Chromatin Modifications Required to Activate rRNA Gene Transcription and Cell Cycle Progression. In: PLoS Genetics. 2013 ; Vol. 9, No. 3.
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