Nuclear Myosin 1c Facilitates the Chromatin Modifications Required to Activate rRNA Gene Transcription and Cell Cycle Progression

Aishe Sarshad, Fatemeh Sadeghifar, Emilie Louvet, Raffaele Mori, Stefanie Böhm, Bader Al-Muzzaini, Anna Vintermist, Nathalie Fomproix, Ann Kristin Östlund, Piergiorgio Percipalle

    Research output: Contribution to journalArticle

    Abstract

    Actin and nuclear myosin 1c (NM1) cooperate in RNA polymerase I (pol I) transcription. NM1 is also part of a multiprotein assembly, B-WICH, which is involved in transcription. This assembly contains the chromatin remodeling complex WICH with its subunits WSTF and SNF2h. We report here that NM1 binds SNF2h with enhanced affinity upon impairment of the actin-binding function. ChIP analysis revealed that NM1, SNF2h, and actin gene occupancies are cell cycle-dependent and require intact motor function. At the onset of cell division, when transcription is temporarily blocked, B-WICH is disassembled due to WSTF phosphorylation, to be reassembled on the active gene at exit from mitosis. NM1 gene knockdown and motor function inhibition, or stable expression of NM1 mutants that do not interact with actin or chromatin, overall repressed rRNA synthesis by stalling pol I at the gene promoter, led to chromatin alterations by changing the state of H3K9 acetylation at gene promoter, and delayed cell cycle progression. These results suggest a unique structural role for NM1 in which the interaction with SNF2h stabilizes B-WICH at the gene promoter and facilitates recruitment of the HAT PCAF. This leads to a permissive chromatin structure required for transcription activation.

    Original languageEnglish (US)
    Article numbere1003397
    JournalPLoS Genetics
    Volume9
    Issue number3
    DOIs
    StatePublished - Apr 15 2013

    Fingerprint

    Myosins
    myosin
    rRNA Genes
    Chromatin
    chromatin
    cell cycle
    Cell Cycle
    transcription (genetics)
    ribosomal RNA
    gene
    actin
    Actins
    genes
    promoter regions
    Genes
    RNA Polymerase I
    Gene Knockdown Techniques
    cdc Genes
    Chromatin Assembly and Disassembly
    acetylation

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Molecular Biology
    • Genetics
    • Genetics(clinical)
    • Cancer Research

    Cite this

    Sarshad, A., Sadeghifar, F., Louvet, E., Mori, R., Böhm, S., Al-Muzzaini, B., ... Percipalle, P. (2013). Nuclear Myosin 1c Facilitates the Chromatin Modifications Required to Activate rRNA Gene Transcription and Cell Cycle Progression. PLoS Genetics, 9(3), [e1003397]. https://doi.org/10.1371/journal.pgen.1003397

    Nuclear Myosin 1c Facilitates the Chromatin Modifications Required to Activate rRNA Gene Transcription and Cell Cycle Progression. / Sarshad, Aishe; Sadeghifar, Fatemeh; Louvet, Emilie; Mori, Raffaele; Böhm, Stefanie; Al-Muzzaini, Bader; Vintermist, Anna; Fomproix, Nathalie; Östlund, Ann Kristin; Percipalle, Piergiorgio.

    In: PLoS Genetics, Vol. 9, No. 3, e1003397, 15.04.2013.

    Research output: Contribution to journalArticle

    Sarshad, A, Sadeghifar, F, Louvet, E, Mori, R, Böhm, S, Al-Muzzaini, B, Vintermist, A, Fomproix, N, Östlund, AK & Percipalle, P 2013, 'Nuclear Myosin 1c Facilitates the Chromatin Modifications Required to Activate rRNA Gene Transcription and Cell Cycle Progression', PLoS Genetics, vol. 9, no. 3, e1003397. https://doi.org/10.1371/journal.pgen.1003397
    Sarshad, Aishe ; Sadeghifar, Fatemeh ; Louvet, Emilie ; Mori, Raffaele ; Böhm, Stefanie ; Al-Muzzaini, Bader ; Vintermist, Anna ; Fomproix, Nathalie ; Östlund, Ann Kristin ; Percipalle, Piergiorgio. / Nuclear Myosin 1c Facilitates the Chromatin Modifications Required to Activate rRNA Gene Transcription and Cell Cycle Progression. In: PLoS Genetics. 2013 ; Vol. 9, No. 3.
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