Nuclear myosin 1 contributes to a chromatin landscape compatible with RNA polymerase II transcription activation

Bader Almuzzaini, Aishe A. Sarshad, Ann Kristin Östlund Farrants, Piergiorgio Percipalle

    Research output: Contribution to journalArticle

    Abstract

    Background: Nuclear myosin 1c (NM1) is emerging as a regulator of transcription and chromatin organization. Results: Using chromatin immunoprecipitation and deep sequencing (ChIP-Seq) in combination with molecular analyses, we investigated the global association of NM1 with the mammalian genome. Analysis of the ChIP-Seq data demonstrates that NM1 binds across the entire mammalian genome with occupancy peaks correlating with distributions of RNA Polymerase II (Pol II) and active epigenetic marks at class II gene promoters. In mouse embryonic fibroblasts subjected to RNAi mediated NM1 gene silencing, we show that NM1 synergizes with polymerase-associated actin to maintain active Pol II at the promoter. NM1 also co-localizes with the nucleosome remodeler SNF2h at class II promoters where they assemble together with WSTF as part of the B-WICH complex. A high resolution micrococcal nuclease (MNase) assay and quantitative real time PCR shows that this mechanism is required for local chromatin remodeling. Following B-WICH assembly, NM1 mediates physical recruitment of the histone acetyl transferase PCAF and the histone methyl transferase Set1/Ash2 to maintain and preserve H3K9acetylation and H3K4trimethylation for active transcription. Conclusions: We propose a novel genome-wide mechanism where myosin synergizes with Pol II-associated actin to link the polymerase machinery with permissive chromatin for transcription activation.

    Original languageEnglish (US)
    Article number35
    JournalBMC Biology
    Volume13
    Issue number1
    DOIs
    StatePublished - Dec 12 2015

    Fingerprint

    RNA Polymerase II
    transcriptional activation
    Transcription
    DNA-directed RNA polymerase
    Myosins
    myosin
    Transcriptional Activation
    Chromatin
    chromatin
    RNA
    genome
    Chemical activation
    Genes
    gene
    High-Throughput Nucleotide Sequencing
    machinery
    Chromatin Immunoprecipitation
    promoter regions
    Genome
    Transferases

    Keywords

    • Epigenetics
    • Genome-wide analysis
    • NM1
    • RNA polymerase II transcription

    ASJC Scopus subject areas

    • Biotechnology
    • Structural Biology
    • Physiology
    • Ecology, Evolution, Behavior and Systematics
    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)
    • Plant Science
    • Developmental Biology
    • Cell Biology

    Cite this

    Nuclear myosin 1 contributes to a chromatin landscape compatible with RNA polymerase II transcription activation. / Almuzzaini, Bader; Sarshad, Aishe A.; Farrants, Ann Kristin Östlund; Percipalle, Piergiorgio.

    In: BMC Biology, Vol. 13, No. 1, 35, 12.12.2015.

    Research output: Contribution to journalArticle

    Almuzzaini, Bader ; Sarshad, Aishe A. ; Farrants, Ann Kristin Östlund ; Percipalle, Piergiorgio. / Nuclear myosin 1 contributes to a chromatin landscape compatible with RNA polymerase II transcription activation. In: BMC Biology. 2015 ; Vol. 13, No. 1.
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