Nuclear actin is associated with a specific subset of hnRNP A/B-type proteins

Piergiorgio Percipalle, Andreas Jonsson, Dmitri Nashchekin, Christina Karlsson, Tomas Bergman, Apostolia Guialis, Bertil Daneholt

Research output: Contribution to journalArticle

Abstract

Pre-mRNP complexes were isolated from rat liver nuclei as 40S hnRNP particles, and actin-binding proteins were collected by DNase I affinity chromatography. The bound proteins were analyzed by 2D gel electrophoresis, and the following five hnRNP A/B-type proteins were identified by tandem mass spectrometry: DBP40/CBF-A (CArG binding factor A), a minor hnRNP A2 variant and three minor hnRNP A3 (mBx) variants. DBP40 was chosen for further analysis of the association of actin with the pre-mRNP complex. It was shown in vitro that purified actin binds to recombinant DBP40 suggesting that the interaction between actin and DBP40 is direct in the pre-mRNP particles. The association of actin with DBP40 was further explored in vivo. It was shown in a transfection study that DBP40 appears both in the nucleus and cytoplasm. Microinjection experiments revealed that DBP40 is exported from the nucleus to the cytoplasm. Finally, RNA-protein and protein-protein cross-linking experiments showed that DBP40 interacts with poly(A)+ RNA as well as actin, both in the nucleus and cytoplasm. We propose that actin associated with DBP40, and perhaps with additional hnRNP A/B-type proteins, is transferred from nucleus to cytoplasm bound to mRNA.

Original languageEnglish (US)
Pages (from-to)1725-1734
Number of pages10
JournalNucleic Acids Research
Volume30
Issue number8
StatePublished - Apr 15 2002

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Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Actins
Cytoplasm
Heterogeneous-Nuclear Ribonucleoproteins
Proteins
cat hnRNP A-B related protein
Microfilament Proteins
Messenger RNA
Deoxyribonuclease I
Electrophoresis, Gel, Two-Dimensional
Microinjections
Tandem Mass Spectrometry
Affinity Chromatography
Transfection

ASJC Scopus subject areas

  • Genetics

Cite this

Percipalle, P., Jonsson, A., Nashchekin, D., Karlsson, C., Bergman, T., Guialis, A., & Daneholt, B. (2002). Nuclear actin is associated with a specific subset of hnRNP A/B-type proteins. Nucleic Acids Research, 30(8), 1725-1734.

Nuclear actin is associated with a specific subset of hnRNP A/B-type proteins. / Percipalle, Piergiorgio; Jonsson, Andreas; Nashchekin, Dmitri; Karlsson, Christina; Bergman, Tomas; Guialis, Apostolia; Daneholt, Bertil.

In: Nucleic Acids Research, Vol. 30, No. 8, 15.04.2002, p. 1725-1734.

Research output: Contribution to journalArticle

Percipalle, P, Jonsson, A, Nashchekin, D, Karlsson, C, Bergman, T, Guialis, A & Daneholt, B 2002, 'Nuclear actin is associated with a specific subset of hnRNP A/B-type proteins', Nucleic Acids Research, vol. 30, no. 8, pp. 1725-1734.
Percipalle P, Jonsson A, Nashchekin D, Karlsson C, Bergman T, Guialis A et al. Nuclear actin is associated with a specific subset of hnRNP A/B-type proteins. Nucleic Acids Research. 2002 Apr 15;30(8):1725-1734.
Percipalle, Piergiorgio ; Jonsson, Andreas ; Nashchekin, Dmitri ; Karlsson, Christina ; Bergman, Tomas ; Guialis, Apostolia ; Daneholt, Bertil. / Nuclear actin is associated with a specific subset of hnRNP A/B-type proteins. In: Nucleic Acids Research. 2002 ; Vol. 30, No. 8. pp. 1725-1734.
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