Novel response to microtubule perturbation in meiosis

Andreas Hochwagen, Gunnar Wrobel, Marie Cartron, Philippe Demougin, Christa Niederhauser-Wiederkehr, Monica G. Boselli, Michael Primig, Angelika Amon

Research output: Contribution to journalArticle

Abstract

During the mitotic cell cycle, microtubule depolymerization leads to a cell cycle arrest in metaphase, due to activation of the spindle checkpoint. Here, we show that under microtubule-destabilizing conditions, such as low temperature or the presence of the spindle-depolymerizing drug benomyl, meiotic budding yeast cells arrest in G1 or G2, instead of metaphase. Cells arrest in G1 if microtubule perturbation occurs as they enter the meiotic cell cycle and in G2 if cells are already undergoing premeiotic S phase. Concomitantly, cells down-regulate genes required for cell cycle progression, meiotic differentiation, and spore formation in a highly coordinated manner. Decreased expression of these genes is likely to be responsible for halting both cell cycle progression and meiotic development. Our results point towards the existence of a novel surveillance mechanism of microtubule integrity that may be particularly important during specialized cell cycles when coordination of cell cycle progression with a developmental program is necessary.

Original languageEnglish (US)
Pages (from-to)4767-4781
Number of pages15
JournalMolecular and Cellular Biology
Volume25
Issue number11
DOIs
StatePublished - Jun 2005

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Meiosis
Microtubules
Cell Cycle
Metaphase
Benomyl
cdc Genes
Saccharomycetales
Cell Cycle Checkpoints
Spores
S Phase
Down-Regulation
Gene Expression
Temperature
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Hochwagen, A., Wrobel, G., Cartron, M., Demougin, P., Niederhauser-Wiederkehr, C., Boselli, M. G., ... Amon, A. (2005). Novel response to microtubule perturbation in meiosis. Molecular and Cellular Biology, 25(11), 4767-4781. https://doi.org/10.1128/MCB.25.11.4767-4781.2005

Novel response to microtubule perturbation in meiosis. / Hochwagen, Andreas; Wrobel, Gunnar; Cartron, Marie; Demougin, Philippe; Niederhauser-Wiederkehr, Christa; Boselli, Monica G.; Primig, Michael; Amon, Angelika.

In: Molecular and Cellular Biology, Vol. 25, No. 11, 06.2005, p. 4767-4781.

Research output: Contribution to journalArticle

Hochwagen, A, Wrobel, G, Cartron, M, Demougin, P, Niederhauser-Wiederkehr, C, Boselli, MG, Primig, M & Amon, A 2005, 'Novel response to microtubule perturbation in meiosis', Molecular and Cellular Biology, vol. 25, no. 11, pp. 4767-4781. https://doi.org/10.1128/MCB.25.11.4767-4781.2005
Hochwagen A, Wrobel G, Cartron M, Demougin P, Niederhauser-Wiederkehr C, Boselli MG et al. Novel response to microtubule perturbation in meiosis. Molecular and Cellular Biology. 2005 Jun;25(11):4767-4781. https://doi.org/10.1128/MCB.25.11.4767-4781.2005
Hochwagen, Andreas ; Wrobel, Gunnar ; Cartron, Marie ; Demougin, Philippe ; Niederhauser-Wiederkehr, Christa ; Boselli, Monica G. ; Primig, Michael ; Amon, Angelika. / Novel response to microtubule perturbation in meiosis. In: Molecular and Cellular Biology. 2005 ; Vol. 25, No. 11. pp. 4767-4781.
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