Novel modes of localization and function of nanos in the wasp Nasonia

Jeremy A. Lynch, Claude Desplan

Research output: Contribution to journalArticle

Abstract

Abdominal patterning in Drosophila requires the function of nanos (nos) to prevent translation of hunchback (hb) mRNA in the posterior of the embryo. nos function is restricted to the posterior by the translational repression of mRNA that is not incorporated into the posteriorly localized germ plasm during oogenesis. The wasp Nasonia vitripennis (Nv) undergoes a long germ mode of development very similar to Drosophila, although the molecular patterning mechanisms employed in these two organisms have diverged significantly, reflecting the independent evolution of this mode of development. Here, we report that although Nv nanos (Nv-nos) has a conserved function in embryonic patterning through translational repression of hb, the timing and mechanisms of this repression are significantly delayed in the wasp compared with the fly. This delay in Nv-nos function appears to be related to the dynamic behavior of the germ plasm in Nasonia, as well as to the maternal provision of Nv-Hb protein during oogenesis. Unlike in flies, there appears to be two functional populations of Nv-nos mRNA: one that is concentrated in the oosome and is taken up into the pole cells before evidence of Nv-hb repression is observed; another that forms a gradient at the posterior and plays a role in Nv-hb translational repression. Altogether, our results show that, although the embryonic patterning function of nos orthologs is broadly conserved, the mechanisms employed to achieve this function are distinct.

Original languageEnglish (US)
Pages (from-to)3813-3821
Number of pages9
JournalDevelopment
Volume137
Issue number22
DOIs
StatePublished - Nov 15 2010

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Oogenesis
Wasps
Diptera
Drosophila
Messenger RNA
Protein Biosynthesis
Embryonic Structures
Mothers
Population
Proteins

Keywords

  • Evo-devo
  • Germ plasm
  • Nanos
  • Nasonia
  • Patterning

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Novel modes of localization and function of nanos in the wasp Nasonia. / Lynch, Jeremy A.; Desplan, Claude.

In: Development, Vol. 137, No. 22, 15.11.2010, p. 3813-3821.

Research output: Contribution to journalArticle

Lynch, Jeremy A. ; Desplan, Claude. / Novel modes of localization and function of nanos in the wasp Nasonia. In: Development. 2010 ; Vol. 137, No. 22. pp. 3813-3821.
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