Nonphotochemical laser induced nucleation of hen egg white lysozyme crystal

In Sung Lee, James M B Evans, Deniz Erdemir, Alfred Y. Lee, Bruce Garetz, Allan S. Myerson

Research output: Contribution to journalArticle

Abstract

Small droplets of supersaturated hen egg white lysozyme (HEWL) solution were exposed to intense linearly polarized laser pulses with different wavelengths, intensities, and pulse durations. Laser irradiation under some conditions significantly increased the number of droplets in which crystals were observed in a given time period, compared with nonirradiated samples. As a general rule, nonphotochemical laser induced nucleation (NPLIN) in lysozyme solutions was more effective with shorter aging time, 532-nm wavelength, higher peak intensity, and shorter pulse duration. Bovine pancreatic trypsin (BPT) was also examined using NPLIN, showing the potential application of NPLIN to other proteins.

Original languageEnglish (US)
Pages (from-to)4255-4261
Number of pages7
JournalCrystal Growth and Design
Volume8
Issue number12
DOIs
StatePublished - Dec 2008

Fingerprint

eggs
lysozyme
Muramidase
Nucleation
Enzymes
nucleation
Crystals
Lasers
Laser pulses
crystals
lasers
Wavelength
Laser beam effects
pulse duration
Trypsin
trypsin
Aging of materials
Proteins
wavelengths
proteins

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Lee, I. S., Evans, J. M. B., Erdemir, D., Lee, A. Y., Garetz, B., & Myerson, A. S. (2008). Nonphotochemical laser induced nucleation of hen egg white lysozyme crystal. Crystal Growth and Design, 8(12), 4255-4261. https://doi.org/10.1021/cg800696u

Nonphotochemical laser induced nucleation of hen egg white lysozyme crystal. / Lee, In Sung; Evans, James M B; Erdemir, Deniz; Lee, Alfred Y.; Garetz, Bruce; Myerson, Allan S.

In: Crystal Growth and Design, Vol. 8, No. 12, 12.2008, p. 4255-4261.

Research output: Contribution to journalArticle

Lee, IS, Evans, JMB, Erdemir, D, Lee, AY, Garetz, B & Myerson, AS 2008, 'Nonphotochemical laser induced nucleation of hen egg white lysozyme crystal', Crystal Growth and Design, vol. 8, no. 12, pp. 4255-4261. https://doi.org/10.1021/cg800696u
Lee, In Sung ; Evans, James M B ; Erdemir, Deniz ; Lee, Alfred Y. ; Garetz, Bruce ; Myerson, Allan S. / Nonphotochemical laser induced nucleation of hen egg white lysozyme crystal. In: Crystal Growth and Design. 2008 ; Vol. 8, No. 12. pp. 4255-4261.
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