Nonequilibrium phase diagrams for actomyosin networks

Simon L. Freedman, Glen Hocky, Shiladitya Banerjee, Aaron R. Dinner

Research output: Contribution to journalArticle

Abstract

Living cells dynamically modulate the local morphologies of their actin networks to perform biological functions, including force transduction, intracellular transport, and cell division. A major challenge is to understand how diverse structures of the actin cytoskeleton are assembled from a limited set of molecular building blocks. Here we study the spontaneous self-assembly of a minimal model of cytoskeletal materials, consisting of semiflexible actin filaments, crosslinkers, and molecular motors. Using coarse-grained simulations, we demonstrate that by changing concentrations and kinetics of crosslinkers and motors, as well as filament lengths, we can generate three distinct structural phases of actomyosin assemblies: bundled, polarity-sorted, and contracted. We introduce new metrics to distinguish these structural phases and demonstrate their functional roles. We find that the binding kinetics of motors and crosslinkers can be tuned to optimize contractile force generation, motor transport, and mechanical response. By quantitatively characterizing the relationships between the modes of cytoskeletal self-assembly, the resulting structures, and their functional consequences, our work suggests new principles for the design of active materials.

Original languageEnglish (US)
Pages (from-to)7740-7747
Number of pages8
JournalSoft Matter
Volume14
Issue number37
DOIs
StatePublished - Jan 1 2018

Fingerprint

Actomyosin
Phase diagrams
phase diagrams
Actins
Self assembly
self assembly
filaments
Cells
cell division
Kinetics
kinetics
assemblies
polarity
simulation

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

Cite this

Freedman, S. L., Hocky, G., Banerjee, S., & Dinner, A. R. (2018). Nonequilibrium phase diagrams for actomyosin networks. Soft Matter, 14(37), 7740-7747. https://doi.org/10.1039/c8sm00741a

Nonequilibrium phase diagrams for actomyosin networks. / Freedman, Simon L.; Hocky, Glen; Banerjee, Shiladitya; Dinner, Aaron R.

In: Soft Matter, Vol. 14, No. 37, 01.01.2018, p. 7740-7747.

Research output: Contribution to journalArticle

Freedman, SL, Hocky, G, Banerjee, S & Dinner, AR 2018, 'Nonequilibrium phase diagrams for actomyosin networks', Soft Matter, vol. 14, no. 37, pp. 7740-7747. https://doi.org/10.1039/c8sm00741a
Freedman, Simon L. ; Hocky, Glen ; Banerjee, Shiladitya ; Dinner, Aaron R. / Nonequilibrium phase diagrams for actomyosin networks. In: Soft Matter. 2018 ; Vol. 14, No. 37. pp. 7740-7747.
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