NMR studies on the structure/function correlations of T-cell-epitope analogs from pertussis toxin

Maria Scarselli, Gennaro Esposito, M. Teresa De Magistris, Mario Domenighini, Rino Rappuoli, Gigliola Burroni, Andrea Bernini, Neri Niccolai

Research output: Contribution to journalArticle

Abstract

A synthetic tridecapeptide, corresponding to the 30-42 fragment of the S1 subunit of pertussis toxin, has been structurally characterised by using NMR spectroscopy. The molecule corresponds to a T-cell epitope of the bacterial toxin which has been extensively analysed with the alanine scanning approach to check the relevance of each residue for the biological activity of the peptide. Five of these Ala-substituted analogs have also been spectroscopically studied. In the experimental conditions used, different extents of helicity were found for the six peptides in a way which cannot be related to their capabilities of of binding to major histocompatibility complex (MHC) class II and inducing T-cell proliferation. Backbone flexibility around helical transient conformations seems to constitute the structural intermediate step between the structure of the corresponding sequence within the parental protein and in the MHC class II complex. A model of the latter complex, which accounts for the different biological activities of the analogs, is proposed.

Original languageEnglish (US)
Pages (from-to)313-317
Number of pages5
JournalEuropean Journal of Biochemistry
Volume254
Issue number2
DOIs
StatePublished - Jun 1 1998

Fingerprint

T-Lymphocyte Epitopes
Pertussis Toxin
Bioactivity
Major Histocompatibility Complex
Nuclear magnetic resonance
Bacterial Toxins
Peptides
T-cells
Cell proliferation
Alanine
Nuclear magnetic resonance spectroscopy
Conformations
Magnetic Resonance Spectroscopy
Cell Proliferation
Scanning
T-Lymphocytes
Molecules
Proteins
S1 subunit pertussis toxin

Keywords

  • Major-histocompatibility- complex class II
  • NMR
  • Pertussis toxin
  • Structure
  • T-cell epitope

ASJC Scopus subject areas

  • Biochemistry

Cite this

NMR studies on the structure/function correlations of T-cell-epitope analogs from pertussis toxin. / Scarselli, Maria; Esposito, Gennaro; De Magistris, M. Teresa; Domenighini, Mario; Rappuoli, Rino; Burroni, Gigliola; Bernini, Andrea; Niccolai, Neri.

In: European Journal of Biochemistry, Vol. 254, No. 2, 01.06.1998, p. 313-317.

Research output: Contribution to journalArticle

Scarselli, M, Esposito, G, De Magistris, MT, Domenighini, M, Rappuoli, R, Burroni, G, Bernini, A & Niccolai, N 1998, 'NMR studies on the structure/function correlations of T-cell-epitope analogs from pertussis toxin', European Journal of Biochemistry, vol. 254, no. 2, pp. 313-317. https://doi.org/10.1046/j.1432-1327.1998.2540313.x
Scarselli, Maria ; Esposito, Gennaro ; De Magistris, M. Teresa ; Domenighini, Mario ; Rappuoli, Rino ; Burroni, Gigliola ; Bernini, Andrea ; Niccolai, Neri. / NMR studies on the structure/function correlations of T-cell-epitope analogs from pertussis toxin. In: European Journal of Biochemistry. 1998 ; Vol. 254, No. 2. pp. 313-317.
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