NMR studies of toxin III from the sea anemone Radianthus paumotensis and comparison of its secondary structure with related toxins

Joseph H B Pease, N. Vasant Kumar, Hugues Schweitz, Neville R. Kallenbach, David E. Wemmer

Research output: Contribution to journalArticle

Abstract

Nearly complete assignments of the proton nuclear magnetic resonance (NMR) spectrum of the polypeptide toxin III from the sea anemone Radianthus paumotensis (RP) are presented. The secondary structures of the related toxins RP II and RP III are described and are compared with each other and with another related toxin ATX Ia from Ammonia sulcata [Widmer, H., Wagner, G., Schweitz, H., Lazdunski, M., & Wüthrich, K. (1988) Eur. J. Biochem. 171, 177-192]. All of these proteins contain a highly twisted four-strand antiparallel β-sheet core connected by loops of irregular structure. From the work done with AP-A from Anthopleura xanthogrammica [Gooley, P. R., & Norton, R. S. (1986) Biochemistry 25, 2349-2356], it is clear that this homologous toxin also has the same basic core. Some small differences are seen in the structures of these toxins, particularly in the position of the N-terminal residues that form one of the outside strands of the β-sheet. In addition, the R. paumotensis toxins are two residues longer, extending the third strand of sheet containing the C-terminal residues. A comparison of chemical shifts for assigned residues is also presented, in general supporting the similarity of structure among these proteins.

Original languageEnglish (US)
Pages (from-to)2199-2204
Number of pages6
JournalBiochemistry
Volume28
Issue number5
StatePublished - 1989

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Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Biochemistry
Chemical shift
Ammonia
Protons
Proteins
Peptides
toxin III (Anemonia sulcata)

ASJC Scopus subject areas

  • Biochemistry

Cite this

Pease, J. H. B., Kumar, N. V., Schweitz, H., Kallenbach, N. R., & Wemmer, D. E. (1989). NMR studies of toxin III from the sea anemone Radianthus paumotensis and comparison of its secondary structure with related toxins. Biochemistry, 28(5), 2199-2204.

NMR studies of toxin III from the sea anemone Radianthus paumotensis and comparison of its secondary structure with related toxins. / Pease, Joseph H B; Kumar, N. Vasant; Schweitz, Hugues; Kallenbach, Neville R.; Wemmer, David E.

In: Biochemistry, Vol. 28, No. 5, 1989, p. 2199-2204.

Research output: Contribution to journalArticle

Pease, JHB, Kumar, NV, Schweitz, H, Kallenbach, NR & Wemmer, DE 1989, 'NMR studies of toxin III from the sea anemone Radianthus paumotensis and comparison of its secondary structure with related toxins', Biochemistry, vol. 28, no. 5, pp. 2199-2204.
Pease, Joseph H B ; Kumar, N. Vasant ; Schweitz, Hugues ; Kallenbach, Neville R. ; Wemmer, David E. / NMR studies of toxin III from the sea anemone Radianthus paumotensis and comparison of its secondary structure with related toxins. In: Biochemistry. 1989 ; Vol. 28, No. 5. pp. 2199-2204.
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