NMR spectroscopy approach to study the structure, orientation, and mechanism of the multidrug exporter EmrE

Maureen Leninger, Nathaniel Traaseth

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Multidrug exporters are a class of membrane proteins that remove antibiotics from the cytoplasm of bacteria and in the process confer multidrug resistance to the organism. This chapter outlines the sample preparation and optimization of oriented solid-state NMR experiments applied to the study of structure and dynamics for the model transporter EmrE from the small multidrug resistance (SMR) family.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages83-96
Number of pages14
Volume1700
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1700
ISSN (Print)1064-3745

Fingerprint

Multiple Drug Resistance
Magnetic Resonance Spectroscopy
Membrane Proteins
Cytoplasm
Anti-Bacterial Agents
Bacteria

Keywords

  • Bicelles
  • Membrane proteins
  • Multidrug resistance
  • NMR pedagogy
  • PISEMA
  • Solid-state NMR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Leninger, M., & Traaseth, N. (2018). NMR spectroscopy approach to study the structure, orientation, and mechanism of the multidrug exporter EmrE. In Methods in Molecular Biology (Vol. 1700, pp. 83-96). (Methods in Molecular Biology; Vol. 1700). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7454-2_6

NMR spectroscopy approach to study the structure, orientation, and mechanism of the multidrug exporter EmrE. / Leninger, Maureen; Traaseth, Nathaniel.

Methods in Molecular Biology. Vol. 1700 Humana Press Inc., 2018. p. 83-96 (Methods in Molecular Biology; Vol. 1700).

Research output: Chapter in Book/Report/Conference proceedingChapter

Leninger, M & Traaseth, N 2018, NMR spectroscopy approach to study the structure, orientation, and mechanism of the multidrug exporter EmrE. in Methods in Molecular Biology. vol. 1700, Methods in Molecular Biology, vol. 1700, Humana Press Inc., pp. 83-96. https://doi.org/10.1007/978-1-4939-7454-2_6
Leninger M, Traaseth N. NMR spectroscopy approach to study the structure, orientation, and mechanism of the multidrug exporter EmrE. In Methods in Molecular Biology. Vol. 1700. Humana Press Inc. 2018. p. 83-96. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7454-2_6
Leninger, Maureen ; Traaseth, Nathaniel. / NMR spectroscopy approach to study the structure, orientation, and mechanism of the multidrug exporter EmrE. Methods in Molecular Biology. Vol. 1700 Humana Press Inc., 2018. pp. 83-96 (Methods in Molecular Biology).
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