NMR scalar coupling constant reveals that intraprotein hydrogen bonds are dynamically stabilized by electronic polarization

Chang G. Ji, John Zhang

Research output: Contribution to journalArticle

Abstract

Molecular dynamics simulations based on the standard nonpolarizable AMBER force field and on quantumderived polarized protein-specific charge (PPC) are performed to compute NMR scalar coupling constants across hydrogen bonds for three benchmark protein systems: ubiquitin, the GB1 domain of protein G, and the SMN Tudor domain. Direct comparison of the simulation result with experimental data gives strong evidence that intraprotein hydrogen bonds are significantly stabilized by electronic polarization, both in terms of NMR scalar coupling constants and X-ray determined geometries of hydrogen bonds. Without the polarization effect in the force field, hydrogen bonds are found to be "too loose", which leads to less stable or even unstable local structures of proteins.

Original languageEnglish (US)
Pages (from-to)13898-13900
Number of pages3
JournalJournal of Physical Chemistry B
Volume113
Issue number42
DOIs
StatePublished - Oct 22 2009

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Hydrogen bonds
Nuclear magnetic resonance
Polarization
hydrogen bonds
scalars
proteins
Proteins
nuclear magnetic resonance
polarization
electronics
field theory (physics)
Ubiquitin
Molecular dynamics
simulation
molecular dynamics
X rays
Geometry
Computer simulation
geometry
x rays

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Materials Chemistry
  • Surfaces, Coatings and Films

Cite this

NMR scalar coupling constant reveals that intraprotein hydrogen bonds are dynamically stabilized by electronic polarization. / Ji, Chang G.; Zhang, John.

In: Journal of Physical Chemistry B, Vol. 113, No. 42, 22.10.2009, p. 13898-13900.

Research output: Contribution to journalArticle

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