NMR delineation of inner and outer protons from paramagnetic relaxation perturbations in 1D and 2D spectra of peptides

Neri Niccolai, Alessandra Bonci, Mauro Rustici, M. Scarselli, Paolo Neri, Gennaro Esposito, Paolo Mascagni, Andrea Motta, Henríette Molinari

Research output: Contribution to journalArticle

Abstract

The use of soluble spin labels to filter out the cross-peaks of outer proton nuclei in 2D NMR spectra is proposed as a general method of obtaining structural information about complex molecules. Gramicidin S, a decapeptide of well defined and stable structure, has been used as a model system to correlate the paramagnetic effects observed in 1D and 2D spectra and the peptide solution conformation. The method appears particularly suited to obtaining information about the hydrogen bonding of backbone amide protons.

Original languageEnglish (US)
Pages (from-to)1453-1457
Number of pages5
JournalJournal of the Chemical Society, Perkin Transactions 2
Issue number9
StatePublished - Dec 1 1991

Fingerprint

Protons
Nuclear magnetic resonance
Gramicidin
Spin Labels
Peptides
Amides
Conformations
Hydrogen bonds
Molecules

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

NMR delineation of inner and outer protons from paramagnetic relaxation perturbations in 1D and 2D spectra of peptides. / Niccolai, Neri; Bonci, Alessandra; Rustici, Mauro; Scarselli, M.; Neri, Paolo; Esposito, Gennaro; Mascagni, Paolo; Motta, Andrea; Molinari, Henríette.

In: Journal of the Chemical Society, Perkin Transactions 2, No. 9, 01.12.1991, p. 1453-1457.

Research output: Contribution to journalArticle

Niccolai, N, Bonci, A, Rustici, M, Scarselli, M, Neri, P, Esposito, G, Mascagni, P, Motta, A & Molinari, H 1991, 'NMR delineation of inner and outer protons from paramagnetic relaxation perturbations in 1D and 2D spectra of peptides', Journal of the Chemical Society, Perkin Transactions 2, no. 9, pp. 1453-1457.
Niccolai, Neri ; Bonci, Alessandra ; Rustici, Mauro ; Scarselli, M. ; Neri, Paolo ; Esposito, Gennaro ; Mascagni, Paolo ; Motta, Andrea ; Molinari, Henríette. / NMR delineation of inner and outer protons from paramagnetic relaxation perturbations in 1D and 2D spectra of peptides. In: Journal of the Chemical Society, Perkin Transactions 2. 1991 ; No. 9. pp. 1453-1457.
@article{c5de2e9ba4e94993971d26690a92685d,
title = "NMR delineation of inner and outer protons from paramagnetic relaxation perturbations in 1D and 2D spectra of peptides",
abstract = "The use of soluble spin labels to filter out the cross-peaks of outer proton nuclei in 2D NMR spectra is proposed as a general method of obtaining structural information about complex molecules. Gramicidin S, a decapeptide of well defined and stable structure, has been used as a model system to correlate the paramagnetic effects observed in 1D and 2D spectra and the peptide solution conformation. The method appears particularly suited to obtaining information about the hydrogen bonding of backbone amide protons.",
author = "Neri Niccolai and Alessandra Bonci and Mauro Rustici and M. Scarselli and Paolo Neri and Gennaro Esposito and Paolo Mascagni and Andrea Motta and Henr{\'i}ette Molinari",
year = "1991",
month = "12",
day = "1",
language = "English (US)",
pages = "1453--1457",
journal = "Journal of the Chemical Society, Perkin Transactions 2",
issn = "1470-1820",
publisher = "Chemical Society",
number = "9",

}

TY - JOUR

T1 - NMR delineation of inner and outer protons from paramagnetic relaxation perturbations in 1D and 2D spectra of peptides

AU - Niccolai, Neri

AU - Bonci, Alessandra

AU - Rustici, Mauro

AU - Scarselli, M.

AU - Neri, Paolo

AU - Esposito, Gennaro

AU - Mascagni, Paolo

AU - Motta, Andrea

AU - Molinari, Henríette

PY - 1991/12/1

Y1 - 1991/12/1

N2 - The use of soluble spin labels to filter out the cross-peaks of outer proton nuclei in 2D NMR spectra is proposed as a general method of obtaining structural information about complex molecules. Gramicidin S, a decapeptide of well defined and stable structure, has been used as a model system to correlate the paramagnetic effects observed in 1D and 2D spectra and the peptide solution conformation. The method appears particularly suited to obtaining information about the hydrogen bonding of backbone amide protons.

AB - The use of soluble spin labels to filter out the cross-peaks of outer proton nuclei in 2D NMR spectra is proposed as a general method of obtaining structural information about complex molecules. Gramicidin S, a decapeptide of well defined and stable structure, has been used as a model system to correlate the paramagnetic effects observed in 1D and 2D spectra and the peptide solution conformation. The method appears particularly suited to obtaining information about the hydrogen bonding of backbone amide protons.

UR - http://www.scopus.com/inward/record.url?scp=37049088026&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37049088026&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:37049088026

SP - 1453

EP - 1457

JO - Journal of the Chemical Society, Perkin Transactions 2

JF - Journal of the Chemical Society, Perkin Transactions 2

SN - 1470-1820

IS - 9

ER -