NMR Analysis and Sequence of Toxin II from the Sea Anemone Radianthus paumotensis

David E. Wemmer, N. Vasant Kumar, Robert M. Metrione, Michel Lazdunski, Gary Drobny, Neville R. Kallenbach

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Abstract

Toxin II from Radianthus paumotensis (Rpu) has been investigated by high-resolution NMR and chemical sequencing methods. Resonance assignments have been obtained for this protein by the sequential approach. NMR assignments could not be made consistent with the previously reported primary sequence for this protein, and chemical methods have been used to determine a sequence with which the NMR data are consistent. Analysis of the 2D NOE spectra shows that the protein secondary structure is comprised of two sequences of β-sheet, probably joined into a distorted continuous sheet, connected by turns and extended loops, without any regular a-helical segments. The residues previously implicated in activity in this class of proteins, D8 and R13, occur in a loop region.

Original languageEnglish (US)
Pages (from-to)6842-6849
Number of pages8
JournalBiochemistry
Volume25
Issue number22
DOIs
StatePublished - Nov 1986

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ASJC Scopus subject areas

  • Biochemistry

Cite this

Wemmer, D. E., Kumar, N. V., Metrione, R. M., Lazdunski, M., Drobny, G., & Kallenbach, N. R. (1986). NMR Analysis and Sequence of Toxin II from the Sea Anemone Radianthus paumotensis. Biochemistry, 25(22), 6842-6849. https://doi.org/10.1021/bi00370a017