Next-generation sequencing to generate interactome datasets

Haiyuan Yu, Leah Tardivo, Stanley Tam, Evan Weiner, Fana Gebreab, Changyu Fan, Nenad Svrzikapa, Tomoko Hirozane-Kishikawa, Edward Rietman, Xinping Yang, Julie Sahalie, Kourosh Salehi-Ashtiani, Tong Hao, Michael E. Cusick, David E. Hill, Frederick P. Roth, Pascal Braun, Marc Vidal

Research output: Contribution to journalArticle

Abstract

Next-generation sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to various interaction assays and should help expand interactome network mapping.

Original languageEnglish (US)
Pages (from-to)478-480
Number of pages3
JournalNature Methods
Volume8
Issue number6
DOIs
StatePublished - Jun 1 2011

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Proteins
Polymerase Chain Reaction
Assays
Pipelines
Association reactions
Datasets

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yu, H., Tardivo, L., Tam, S., Weiner, E., Gebreab, F., Fan, C., ... Vidal, M. (2011). Next-generation sequencing to generate interactome datasets. Nature Methods, 8(6), 478-480. https://doi.org/10.1038/nmeth.1597

Next-generation sequencing to generate interactome datasets. / Yu, Haiyuan; Tardivo, Leah; Tam, Stanley; Weiner, Evan; Gebreab, Fana; Fan, Changyu; Svrzikapa, Nenad; Hirozane-Kishikawa, Tomoko; Rietman, Edward; Yang, Xinping; Sahalie, Julie; Salehi-Ashtiani, Kourosh; Hao, Tong; Cusick, Michael E.; Hill, David E.; Roth, Frederick P.; Braun, Pascal; Vidal, Marc.

In: Nature Methods, Vol. 8, No. 6, 01.06.2011, p. 478-480.

Research output: Contribution to journalArticle

Yu, H, Tardivo, L, Tam, S, Weiner, E, Gebreab, F, Fan, C, Svrzikapa, N, Hirozane-Kishikawa, T, Rietman, E, Yang, X, Sahalie, J, Salehi-Ashtiani, K, Hao, T, Cusick, ME, Hill, DE, Roth, FP, Braun, P & Vidal, M 2011, 'Next-generation sequencing to generate interactome datasets', Nature Methods, vol. 8, no. 6, pp. 478-480. https://doi.org/10.1038/nmeth.1597
Yu H, Tardivo L, Tam S, Weiner E, Gebreab F, Fan C et al. Next-generation sequencing to generate interactome datasets. Nature Methods. 2011 Jun 1;8(6):478-480. https://doi.org/10.1038/nmeth.1597
Yu, Haiyuan ; Tardivo, Leah ; Tam, Stanley ; Weiner, Evan ; Gebreab, Fana ; Fan, Changyu ; Svrzikapa, Nenad ; Hirozane-Kishikawa, Tomoko ; Rietman, Edward ; Yang, Xinping ; Sahalie, Julie ; Salehi-Ashtiani, Kourosh ; Hao, Tong ; Cusick, Michael E. ; Hill, David E. ; Roth, Frederick P. ; Braun, Pascal ; Vidal, Marc. / Next-generation sequencing to generate interactome datasets. In: Nature Methods. 2011 ; Vol. 8, No. 6. pp. 478-480.
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