New methods for depositing and imaging molecules in scanning tunneling microscopy

V. N. Morozov, N. C. Seeman, N. R. Kalienbach, T. G. Thundat, H. J K Horber, R. Balhorn, S. M. Lindsay, R. L. McCarley

Research output: Contribution to journalArticle

Abstract

Methods and apparatus are described to deposit and image molecules by scanning tunneling microscopy (STM) under an inert atmosphere. Three methods of applying molecules have been evaluated: equilibrium adsorption from the vapor phase, sublimation, and electrospraying. Using these methods, a variety of organic and biopolymer molecules have been deposited and imaged on graphite and on gold (111), grown epitaxially on mica. Compared with alternatives, such as the use of high vacuum apparatus or glove boxes, these procedures offer some important advantages: they are inexpensive, convenient, and more rapid. Mercaptoethanol, ethanolamine, ethanol, acetic acid, and water produce two-dimensional crystalline adlayers on gold substrates, when they are introduced into the scanning cell as vapors. These adlayers are assumed to involve hydrogen bonding of the molecules to an oxide of gold formed on the surface. Electrospraying protein solutions on gold surfaces yielded images of individual protein molecules with lateral dimensions close to those measured by X-ray analysis, and thicknesses of 0.6-1.3 nm. In the case of metallothionein, the known internal domain structure of the molecule was reproducibly observed. No detailed internal structure could be resolved in the other examples examined.

Original languageEnglish (US)
Pages (from-to)757-779
Number of pages23
JournalScanning Microscopy
Volume7
Issue number3
StatePublished - Sep 1993

Fingerprint

Scanning tunneling microscopy
scanning tunneling microscopy
Imaging techniques
Molecules
Gold
gold
molecules
vacuum apparatus
Vapors
gloves
Ethanolamines
proteins
Proteins
inert atmosphere
biopolymers
Sublimation
Biopolymers
Mica
X ray analysis
high vacuum

Keywords

  • Au(111)
  • deposition methods
  • electrospray
  • highly oriented pyrolytic graphite (HOPG)
  • Scanning tunneling microscopy
  • sublimation

ASJC Scopus subject areas

  • Instrumentation

Cite this

Morozov, V. N., Seeman, N. C., Kalienbach, N. R., Thundat, T. G., Horber, H. J. K., Balhorn, R., ... McCarley, R. L. (1993). New methods for depositing and imaging molecules in scanning tunneling microscopy. Scanning Microscopy, 7(3), 757-779.

New methods for depositing and imaging molecules in scanning tunneling microscopy. / Morozov, V. N.; Seeman, N. C.; Kalienbach, N. R.; Thundat, T. G.; Horber, H. J K; Balhorn, R.; Lindsay, S. M.; McCarley, R. L.

In: Scanning Microscopy, Vol. 7, No. 3, 09.1993, p. 757-779.

Research output: Contribution to journalArticle

Morozov, VN, Seeman, NC, Kalienbach, NR, Thundat, TG, Horber, HJK, Balhorn, R, Lindsay, SM & McCarley, RL 1993, 'New methods for depositing and imaging molecules in scanning tunneling microscopy', Scanning Microscopy, vol. 7, no. 3, pp. 757-779.
Morozov VN, Seeman NC, Kalienbach NR, Thundat TG, Horber HJK, Balhorn R et al. New methods for depositing and imaging molecules in scanning tunneling microscopy. Scanning Microscopy. 1993 Sep;7(3):757-779.
Morozov, V. N. ; Seeman, N. C. ; Kalienbach, N. R. ; Thundat, T. G. ; Horber, H. J K ; Balhorn, R. ; Lindsay, S. M. ; McCarley, R. L. / New methods for depositing and imaging molecules in scanning tunneling microscopy. In: Scanning Microscopy. 1993 ; Vol. 7, No. 3. pp. 757-779.
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