Neutrophils migrate longer distances in moving microfluidic concentration gradients compared to static ones

Mohammad Qasaimeh, Mélina Astolfi, Michal Pyzik, Silvia Vidal, David Juncker

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    We present the use of our recently developed microfluidic quadrupole (MQ) to generate controllable microfluidic floating concentration gradients of 25 ng·ml-1 Interleukin 8 (IL-8), and challenge neutrophils cultured in petri-dishes with stationary and moving gradients. The dynamic responses of individual neutrophils were analyzed and we found that the migration length of neutrophils is correlated to their initial position within the stationary gradient. However, for moving gradients this correlation vanishes, while at the same time neutrophils migrate over longer distances. Our results and demonstrated experimentation lay the foundation for future novel chemotaxis assays under moving gradients of different shapes and speeds.

    Original languageEnglish (US)
    Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
    PublisherChemical and Biological Microsystems Society
    Pages2007-2009
    Number of pages3
    Volume3
    ISBN (Print)9781632666246
    StatePublished - Jan 1 2013
    Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
    Duration: Oct 27 2013Oct 31 2013

    Other

    Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
    CountryGermany
    CityFreiburg
    Period10/27/1310/31/13

    Fingerprint

    Microfluidics
    Interleukin-8
    Dynamic response
    Assays

    Keywords

    • Chemotaxis
    • Floating gradients
    • Microfluidic probe
    • Microfluidic quadrupole
    • Neutrophils

    ASJC Scopus subject areas

    • Bioengineering

    Cite this

    Qasaimeh, M., Astolfi, M., Pyzik, M., Vidal, S., & Juncker, D. (2013). Neutrophils migrate longer distances in moving microfluidic concentration gradients compared to static ones. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 3, pp. 2007-2009). Chemical and Biological Microsystems Society.

    Neutrophils migrate longer distances in moving microfluidic concentration gradients compared to static ones. / Qasaimeh, Mohammad; Astolfi, Mélina; Pyzik, Michal; Vidal, Silvia; Juncker, David.

    17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 3 Chemical and Biological Microsystems Society, 2013. p. 2007-2009.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Qasaimeh, M, Astolfi, M, Pyzik, M, Vidal, S & Juncker, D 2013, Neutrophils migrate longer distances in moving microfluidic concentration gradients compared to static ones. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 3, Chemical and Biological Microsystems Society, pp. 2007-2009, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 10/27/13.
    Qasaimeh M, Astolfi M, Pyzik M, Vidal S, Juncker D. Neutrophils migrate longer distances in moving microfluidic concentration gradients compared to static ones. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 3. Chemical and Biological Microsystems Society. 2013. p. 2007-2009
    Qasaimeh, Mohammad ; Astolfi, Mélina ; Pyzik, Michal ; Vidal, Silvia ; Juncker, David. / Neutrophils migrate longer distances in moving microfluidic concentration gradients compared to static ones. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 3 Chemical and Biological Microsystems Society, 2013. pp. 2007-2009
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