Neurotrophic factor for serotogenic neurons prevents degeneration of grafted raphe neurons in the cerebellum

F. C. Zhou, E. C. Azmitia

Research output: Contribution to journalArticle

Abstract

Earlier studies in this laboratory have shown that partial serotonergic (5-HT) deafferentation from the hippocampus induced a homotypic sprouting. The 5-HT-denervated hippocampus was further found to be a favorable environment for the growth of grafted 5-HT, but not for inrepinephrinergic neurons. In the current study, we have extracted a trophic factor in the denervated hippocampus with hypotonic solution in high-speed supernatant fraction. The trophic extract was assayed in a unique experimental paradigm - test the growth of grafted 5-HT neurons in the cerebellum, which receives the least 5-HT innervation, and where grafted 5-HT neurons have seldom survived. The diluted trophic extract (1:200) produced a dramatic increase in (a) the survival rate, (b) the 5-HT fiber-density, and (c) the 5-HT and 5-hydroxyindoleacetic acids lvels of grafted 5-HT neurons in the cerebellum. In addition, Nissl-counterstaining showed that the size of the grafted raphe also apparently increased. The concentrated trophic extract (1:1) did not increase the survival rate of the grafted raphe neurons. These results indicate that (a) the supernatant in the 5-HT-denervated hippocampus contains a potent neurotrophic factor to 5-HT neurons (b) which can either directly or indirectly also support non-5-HT raphe neurons, and (c) possibly an inhibitory factor is present which is detrimental to 5-HT neurons in high concentration.

Original languageEnglish (US)
Pages (from-to)301-308
Number of pages8
JournalBrain Research
Volume507
Issue number2
DOIs
StatePublished - Jan 22 1990

Fingerprint

Nerve Degeneration
Nerve Growth Factors
Cerebellum
Serotonin
Neurons
Hippocampus
Hypotonic Solutions
Hydroxyindoleacetic Acid
Growth

Keywords

  • 5,7-Dihydroxytryptamine
  • 5-Hydroxytryptamine
  • Collateral sprouting
  • Hippocampus
  • Neurotrophic factor
  • Regeneration
  • Transplant

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Neurotrophic factor for serotogenic neurons prevents degeneration of grafted raphe neurons in the cerebellum. / Zhou, F. C.; Azmitia, E. C.

In: Brain Research, Vol. 507, No. 2, 22.01.1990, p. 301-308.

Research output: Contribution to journalArticle

@article{a0d4c2806d634727a1056a6839823063,
title = "Neurotrophic factor for serotogenic neurons prevents degeneration of grafted raphe neurons in the cerebellum",
abstract = "Earlier studies in this laboratory have shown that partial serotonergic (5-HT) deafferentation from the hippocampus induced a homotypic sprouting. The 5-HT-denervated hippocampus was further found to be a favorable environment for the growth of grafted 5-HT, but not for inrepinephrinergic neurons. In the current study, we have extracted a trophic factor in the denervated hippocampus with hypotonic solution in high-speed supernatant fraction. The trophic extract was assayed in a unique experimental paradigm - test the growth of grafted 5-HT neurons in the cerebellum, which receives the least 5-HT innervation, and where grafted 5-HT neurons have seldom survived. The diluted trophic extract (1:200) produced a dramatic increase in (a) the survival rate, (b) the 5-HT fiber-density, and (c) the 5-HT and 5-hydroxyindoleacetic acids lvels of grafted 5-HT neurons in the cerebellum. In addition, Nissl-counterstaining showed that the size of the grafted raphe also apparently increased. The concentrated trophic extract (1:1) did not increase the survival rate of the grafted raphe neurons. These results indicate that (a) the supernatant in the 5-HT-denervated hippocampus contains a potent neurotrophic factor to 5-HT neurons (b) which can either directly or indirectly also support non-5-HT raphe neurons, and (c) possibly an inhibitory factor is present which is detrimental to 5-HT neurons in high concentration.",
keywords = "5,7-Dihydroxytryptamine, 5-Hydroxytryptamine, Collateral sprouting, Hippocampus, Neurotrophic factor, Regeneration, Transplant",
author = "Zhou, {F. C.} and Azmitia, {E. C.}",
year = "1990",
month = "1",
day = "22",
doi = "10.1016/0006-8993(90)90286-K",
language = "English (US)",
volume = "507",
pages = "301--308",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Neurotrophic factor for serotogenic neurons prevents degeneration of grafted raphe neurons in the cerebellum

AU - Zhou, F. C.

AU - Azmitia, E. C.

PY - 1990/1/22

Y1 - 1990/1/22

N2 - Earlier studies in this laboratory have shown that partial serotonergic (5-HT) deafferentation from the hippocampus induced a homotypic sprouting. The 5-HT-denervated hippocampus was further found to be a favorable environment for the growth of grafted 5-HT, but not for inrepinephrinergic neurons. In the current study, we have extracted a trophic factor in the denervated hippocampus with hypotonic solution in high-speed supernatant fraction. The trophic extract was assayed in a unique experimental paradigm - test the growth of grafted 5-HT neurons in the cerebellum, which receives the least 5-HT innervation, and where grafted 5-HT neurons have seldom survived. The diluted trophic extract (1:200) produced a dramatic increase in (a) the survival rate, (b) the 5-HT fiber-density, and (c) the 5-HT and 5-hydroxyindoleacetic acids lvels of grafted 5-HT neurons in the cerebellum. In addition, Nissl-counterstaining showed that the size of the grafted raphe also apparently increased. The concentrated trophic extract (1:1) did not increase the survival rate of the grafted raphe neurons. These results indicate that (a) the supernatant in the 5-HT-denervated hippocampus contains a potent neurotrophic factor to 5-HT neurons (b) which can either directly or indirectly also support non-5-HT raphe neurons, and (c) possibly an inhibitory factor is present which is detrimental to 5-HT neurons in high concentration.

AB - Earlier studies in this laboratory have shown that partial serotonergic (5-HT) deafferentation from the hippocampus induced a homotypic sprouting. The 5-HT-denervated hippocampus was further found to be a favorable environment for the growth of grafted 5-HT, but not for inrepinephrinergic neurons. In the current study, we have extracted a trophic factor in the denervated hippocampus with hypotonic solution in high-speed supernatant fraction. The trophic extract was assayed in a unique experimental paradigm - test the growth of grafted 5-HT neurons in the cerebellum, which receives the least 5-HT innervation, and where grafted 5-HT neurons have seldom survived. The diluted trophic extract (1:200) produced a dramatic increase in (a) the survival rate, (b) the 5-HT fiber-density, and (c) the 5-HT and 5-hydroxyindoleacetic acids lvels of grafted 5-HT neurons in the cerebellum. In addition, Nissl-counterstaining showed that the size of the grafted raphe also apparently increased. The concentrated trophic extract (1:1) did not increase the survival rate of the grafted raphe neurons. These results indicate that (a) the supernatant in the 5-HT-denervated hippocampus contains a potent neurotrophic factor to 5-HT neurons (b) which can either directly or indirectly also support non-5-HT raphe neurons, and (c) possibly an inhibitory factor is present which is detrimental to 5-HT neurons in high concentration.

KW - 5,7-Dihydroxytryptamine

KW - 5-Hydroxytryptamine

KW - Collateral sprouting

KW - Hippocampus

KW - Neurotrophic factor

KW - Regeneration

KW - Transplant

UR - http://www.scopus.com/inward/record.url?scp=0025017884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025017884&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(90)90286-K

DO - 10.1016/0006-8993(90)90286-K

M3 - Article

VL - 507

SP - 301

EP - 308

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -