Neuro-glial neurotrophic interaction in the S-100 beta retarded mutant mouse (Polydactyly Nagoya). III. Transplantation study

S. Ueda, M. Aikawa, M. Kawata, I. Naruse, P. M. Whitaker-Azmitia, E. C. Azmitia

Research output: Contribution to journalArticle

Abstract

The hippocampus and caudo-dorsal cortex of the homozygote of polydactyly mutant mouse (Polydactyly Nagoya, Pdn/Pdn) were markedly reduced in S-100 beta positive astrocytes and serotonergic fibers as compared to the heterozygote (Pdn/+) and wild type (+/+) [39]. The Pdn/Pdn mice die within 2 days after birth, so it is impossible to examine postnatal changes. To demonstrate the developmental change of Pdn/Pdn hippocampal tissue, we transplanted hippocampal pieces of neonatal Pdn/Pdn and +/+ mice into the right and left hippocampus of the same adult +/+ mice, respectively, and immunocytochemically examined them. Two weeks after transplantation, +/+ hippocampal tissue contained a large number of glial fibrillary acidic protein (GFAP) and S-100 beta positive astrocytes and a number of serotonergic fibers. While Pdn/Pdn hippocampal tissue contained numerous GFAP positive astrocytes, S-100 beta positive astrocytes and serotonergic fibers were not observed. Two months after transplantation, GFAP and S-100 beta were expressed in the Pdn/Pdn hippocampal tissue similar to the +/+ tissue. Serotonergic fibers were distributed in the +/+ tissue, while no serotonergic fibers were observed in the Pdn/Pdn transplant tissue. In contrast, no difference was observed in the tyrosine hydroxylase positive fibers between Pdn/Pdn and +/+ grafts. The expression of 5-HT1A receptor-like immunoreactivity was higher in the +/+ tissue than that of Pdn/Pdn tissue. The present results suggest that the expression of S-100 beta in the astrocytes of early stage of transplantation is a critical for fiber ingrowth of serotonergic neurons and expressions of 5-HT1A receptor.
Original languageUndefined
Pages (from-to)15-23
Number of pages9
JournalBrain Research
Volume738
Issue number1
StatePublished - 1996

Keywords

  • Animals Animals, Newborn/physiology Brain/metabolism/pathology/*physiopathology Glial Fibrillary Acidic Protein/metabolism Immunohistochemistry Mice Mice, Mutant Strains Nerve Fibers/metabolism Nerve Tissue/transplantation Neuroglia/*physiology Neurons/*physiology Polydactyly/*genetics/pathology/*physiopathology S100 Proteins/*deficiency/metabolism Serotonin/metabolism Time Factors

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