Neuro-glial neurotrophic interaction in the S-100β retarded mutant mouse (Polydactyly Nagoya). II. Co-cultures study

Shuichi Ueda, Xia P. Hou, Patricia M. Whitaker-Azmitia, Efrain C. Azmitia

Research output: Contribution to journalArticle

Abstract

The homozygote of a mouse strain with genetic polydactyly (Polydactyly Nagoya, Pdn) shows several brain abnormalities, and significant decrease of S-100β in the brain [17]. An accompanying paper [18] demonstrates that the hippocampus and caudo-dorsal cortex of homozygote (Pdn/Pdn) mouse were markedly reduced in S-100β positive astrocytes and serotonergic fibers, and the content of 5-HT and 5-HIAA of hippocampus and cortex of Pdn/Pdn mouse was lower than those of heterozygote (Pdn/+) or wild type (+/+) mice. To further clarify the effects of target tissues from different type brains on the development of serotonergic neurons, raphe neurons from Pdn/Pdn or +/+ newborn mice were co-cultured with hippocampus or cortex of +/+ or Pdn/Pdn newborn mice. The growth of the serotonergic neurons in the mesencephalic raphe tissue dissociated cultures was estimated by measuring the specific uptake of [3H]5-HT. The development of both genotypes (Pdn/Pdn and +/+) of serotonergic neurons was enhanced by co-cultures with target tissues (hippocampus and cortex) of +/+ brain. This effect was not observed in the co-cultures with Pdn/Pdn brain as a source of target tissue. The present results support the idea that the developmental defect of serotonergic fibers in the Pdn mutant mouse is caused by the deficiency of S-100β in the astrocyte of this mutant, and suggest that S-100β is a serotonergic growth factor. This mutant mouse is a useful in vivo model to study neural-glial neurotrophic interactions.

Original languageEnglish (US)
Pages (from-to)284-288
Number of pages5
JournalBrain Research
Volume633
Issue number1-2
DOIs
StatePublished - Jan 7 1994

Fingerprint

Polydactyly
Coculture Techniques
Neuroglia
Serotonergic Neurons
Hippocampus
Brain
Homozygote
Astrocytes
Serotonin
Hydroxyindoleacetic Acid

Keywords

  • Cell culture
  • Growth factor
  • Mutant mouse
  • Neuro-glia interaction
  • S-100β
  • Serotonin

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Neuro-glial neurotrophic interaction in the S-100β retarded mutant mouse (Polydactyly Nagoya). II. Co-cultures study. / Ueda, Shuichi; Hou, Xia P.; Whitaker-Azmitia, Patricia M.; Azmitia, Efrain C.

In: Brain Research, Vol. 633, No. 1-2, 07.01.1994, p. 284-288.

Research output: Contribution to journalArticle

Ueda, Shuichi ; Hou, Xia P. ; Whitaker-Azmitia, Patricia M. ; Azmitia, Efrain C. / Neuro-glial neurotrophic interaction in the S-100β retarded mutant mouse (Polydactyly Nagoya). II. Co-cultures study. In: Brain Research. 1994 ; Vol. 633, No. 1-2. pp. 284-288.
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