Neuro-glial neurotrophic interaction in the S-100β retarded mutant mouse (Polydactyly Nagoya). III. Transplantation study

Shuichi Ueda, Masuo Aikawa, Mitsuhiro Kawata, Ichiro Naruse, Patricia M. Whitaker-Azmitia, Efrain C. Azmitia

Research output: Contribution to journalArticle

Abstract

The hippocampus and caudo-dorsal cortex of the homozygote of polydactyly mutant mouse (Polydactyly Nagoya, Pdn/Pdn) were markedly reduced in S-100β positive astrocytes and serotonergic fibers as compared to the heterozygote (Pdn/+) and wild type (+/+). The Pdn/Pdn mice die within 2 days after birth, so it is impossible to examine postnatal changes. To demonstrate the developmental change of Pdn/Pdn hippocampal tissue, we transplanted hippocampal pieces of neonatal Pdn/Pdn and +/+ mice into the right and left hippocampus of the same adult +/+ mice, respectively, and immunocytochemically examined them. Two weeks after transplantation, +/+ hippocampal tissue contained a large number of glial fibrillary acidic protein (GFAP) and S-100β positive astrocytes and a number of serotonergic fibers. While Pdn/Pdn hippocampal tissue contained numerous GFAP positive astrocytes, S-100β positive astrocytes and serotonergic fibers were not observed. Two months after transplantation, GFAP and S-100β were expressed in the Pdn/Pdn hippocampal tissue similar to the +/+ tissue. Serotonergic fibers were distributed in the +/+ tissue, while no serotonergic fibers were observed in the Pdn/Pdn transplant tissue. In contrast, no difference was observed in the tyrosine hydroxylase positive fibers between Pdn/Pdn and +/+ grafts. The expression of 5-HT1A receptor-like immunoreactivity was higher in the +/+ tissue than that of Pdn/Pdn tissue. The present results suggest that the expression of S-100β in the astrocytes of early stage of transplantation is a critical event for fiber ingrowth of serotonergic neurons and expression of 5-HT1A receptor.

Original languageEnglish (US)
Pages (from-to)15-23
Number of pages9
JournalBrain Research
Volume738
Issue number1
DOIs
StatePublished - Oct 28 1996

Fingerprint

Polydactyly
Neuroglia
Transplantation
Astrocytes
Glial Fibrillary Acidic Protein
Receptor, Serotonin, 5-HT1A
Protein S
Hippocampus
Transplants
Serotonergic Neurons
Tissue Transplantation
Homozygote
Tyrosine 3-Monooxygenase
Heterozygote
Parturition

Keywords

  • 5-HT(1A)
  • immunocytochemistry
  • mutant mouse
  • neuro-glia interaction
  • S-100β
  • serotonin
  • transplantation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neuro-glial neurotrophic interaction in the S-100β retarded mutant mouse (Polydactyly Nagoya). III. Transplantation study. / Ueda, Shuichi; Aikawa, Masuo; Kawata, Mitsuhiro; Naruse, Ichiro; Whitaker-Azmitia, Patricia M.; Azmitia, Efrain C.

In: Brain Research, Vol. 738, No. 1, 28.10.1996, p. 15-23.

Research output: Contribution to journalArticle

Ueda, Shuichi ; Aikawa, Masuo ; Kawata, Mitsuhiro ; Naruse, Ichiro ; Whitaker-Azmitia, Patricia M. ; Azmitia, Efrain C. / Neuro-glial neurotrophic interaction in the S-100β retarded mutant mouse (Polydactyly Nagoya). III. Transplantation study. In: Brain Research. 1996 ; Vol. 738, No. 1. pp. 15-23.
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AB - The hippocampus and caudo-dorsal cortex of the homozygote of polydactyly mutant mouse (Polydactyly Nagoya, Pdn/Pdn) were markedly reduced in S-100β positive astrocytes and serotonergic fibers as compared to the heterozygote (Pdn/+) and wild type (+/+). The Pdn/Pdn mice die within 2 days after birth, so it is impossible to examine postnatal changes. To demonstrate the developmental change of Pdn/Pdn hippocampal tissue, we transplanted hippocampal pieces of neonatal Pdn/Pdn and +/+ mice into the right and left hippocampus of the same adult +/+ mice, respectively, and immunocytochemically examined them. Two weeks after transplantation, +/+ hippocampal tissue contained a large number of glial fibrillary acidic protein (GFAP) and S-100β positive astrocytes and a number of serotonergic fibers. While Pdn/Pdn hippocampal tissue contained numerous GFAP positive astrocytes, S-100β positive astrocytes and serotonergic fibers were not observed. Two months after transplantation, GFAP and S-100β were expressed in the Pdn/Pdn hippocampal tissue similar to the +/+ tissue. Serotonergic fibers were distributed in the +/+ tissue, while no serotonergic fibers were observed in the Pdn/Pdn transplant tissue. In contrast, no difference was observed in the tyrosine hydroxylase positive fibers between Pdn/Pdn and +/+ grafts. The expression of 5-HT1A receptor-like immunoreactivity was higher in the +/+ tissue than that of Pdn/Pdn tissue. The present results suggest that the expression of S-100β in the astrocytes of early stage of transplantation is a critical event for fiber ingrowth of serotonergic neurons and expression of 5-HT1A receptor.

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