Network heterogeneity regulates steering in actin-based motility /631/80/128/1276 /631/80/84/1756 article

Rajaa Boujemaa-Paterski, Cristian Suarez, Tobias Klar, Jie Zhu, Christophe Guérin, Alexander Mogilner, Manuel Théry, Laurent Blanchoin

Research output: Contribution to journalArticle

Abstract

The growth of branched actin networks powers cell-edge protrusions and motility. A heterogeneous density of actin, which yields to a tunable cellular response, characterizes these dynamic structures. We study how actin organization controls both the rate and the steering during lamellipodium growth. We use a high-resolution surface structuration assay combined with mathematical modeling to describe the growth of a reconstituted lamellipodium. We demonstrate that local monomer depletion at the site of assembly negatively impacts the network growth rate. At the same time, network architecture tunes the protrusion efficiency, and regulates the rate of growth. One consequence of this interdependence between monomer depletion and network architecture effects is the ability of heterogeneous network to impose steering during motility. Therefore, we have established that the general principle, by which the cell can modulate the rate and the direction of a protrusion, is by varying both density and architecture of its actin network.

Original languageEnglish (US)
Article number655
JournalNature Communications
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2017

Fingerprint

locomotion
Actins
Growth
Network architecture
Pseudopodia
Monomers
Heterogeneous networks
depletion
monomers
Assays
cells
assembly
high resolution

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Network heterogeneity regulates steering in actin-based motility /631/80/128/1276 /631/80/84/1756 article. / Boujemaa-Paterski, Rajaa; Suarez, Cristian; Klar, Tobias; Zhu, Jie; Guérin, Christophe; Mogilner, Alexander; Théry, Manuel; Blanchoin, Laurent.

In: Nature Communications, Vol. 8, No. 1, 655, 01.12.2017.

Research output: Contribution to journalArticle

Boujemaa-Paterski, Rajaa ; Suarez, Cristian ; Klar, Tobias ; Zhu, Jie ; Guérin, Christophe ; Mogilner, Alexander ; Théry, Manuel ; Blanchoin, Laurent. / Network heterogeneity regulates steering in actin-based motility /631/80/128/1276 /631/80/84/1756 article. In: Nature Communications. 2017 ; Vol. 8, No. 1.
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