Near quantitative production of molecular nitrogen from metabolism of dimethylnitrosamine

S. Milstein, Joseph Guttenplan

Research output: Contribution to journalArticle

Abstract

Molecular nitrogen is produced stoichiometrically from the spontaneous decomposition of N-methyl-N-nitrosourea in phosphate buffer and is readily detected by gas chromatography. It is also generated in high yield from the oxidation of dimethylnitrosamine by mouse or rat liver microsomes. Gas chromatographic analysis for nitrogen generated from dimethylnitrosamine was more difficult because the amount of nitrogen generated was similar to that produced in control tubes which contained no dimethylnitrosamine.

Original languageEnglish (US)
Pages (from-to)337-342
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume87
Issue number1
DOIs
StatePublished - Mar 15 1979

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Dimethylnitrosamine
Metabolism
Nitrogen
Gas chromatography
Gas Chromatography
Methylnitrosourea
Liver Microsomes
Liver
Rats
Buffers
Phosphates
Decomposition
Oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Near quantitative production of molecular nitrogen from metabolism of dimethylnitrosamine. / Milstein, S.; Guttenplan, Joseph.

In: Biochemical and Biophysical Research Communications, Vol. 87, No. 1, 15.03.1979, p. 337-342.

Research output: Contribution to journalArticle

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