NAR breakthrough article DNA-Assisted oligomerization of pore-forming toxin monomers into precisely-controlled protein channels

Anja Henning-Knechtel, Johann Knechtel, Mazin Magzoub

    Research output: Contribution to journalArticle

    Abstract

    We have developed a novel approach for creating membrane-spanning protein-based pores. The construction principle is based on using well-defined, circular DNA nanostructures to arrange a precise number of pore-forming protein toxin monomers. We can thereby obtain, for the first time, protein pores with specifically set diameters. We demonstrate this principle by constructing artificial alpha-hemolysin (αHL) pores. The DNA/αHL hybrid nanopores composed of twelve, twenty or twenty-six monomers show stable insertions into lipid bilayers during electrical recordings, along with steady, pore sizedependent current levels. Our approach successfully advances the applicability of nanopores, in particular towards label-free studies of single molecules in large nanoscaled biological structures.

    Original languageEnglish (US)
    Pages (from-to)12057-12068
    Number of pages12
    JournalNucleic Acids Research
    Volume45
    Issue number21
    DOIs
    StatePublished - Dec 1 2017

    Fingerprint

    Nanopores
    Porins
    Hemolysin Proteins
    Circular DNA
    Nanostructures
    DNA
    Lipid Bilayers
    Membrane Proteins
    Proteins

    ASJC Scopus subject areas

    • Genetics

    Cite this

    NAR breakthrough article DNA-Assisted oligomerization of pore-forming toxin monomers into precisely-controlled protein channels. / Henning-Knechtel, Anja; Knechtel, Johann; Magzoub, Mazin.

    In: Nucleic Acids Research, Vol. 45, No. 21, 01.12.2017, p. 12057-12068.

    Research output: Contribution to journalArticle

    Henning-Knechtel, Anja ; Knechtel, Johann ; Magzoub, Mazin. / NAR breakthrough article DNA-Assisted oligomerization of pore-forming toxin monomers into precisely-controlled protein channels. In: Nucleic Acids Research. 2017 ; Vol. 45, No. 21. pp. 12057-12068.
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