Nanotopography influences adhesion, spreading, and self-renewal of Human embryonic stem cells

Weiqiang Chen, Luis G. Villa-Diaz, Yubing Sun, Shinuo Weng, Jin Koo Kim, Raymond H W Lam, Lin Han, Rong Fan, Paul H. Krebsbach, Jianping Fu

Research output: Contribution to journalArticle

Abstract

Human embryonic stem cells (hESCs) have great potentials for future cell-based therapeutics. However, their mechanosensitivity to biophysical signals from the cellular microenvironment is not well characterized. Here we introduced an effective microfabrication strategy for accurate control and patterning of nanoroughness on glass surfaces. Our results demonstrated that nanotopography could provide a potent regulatory signal over different hESC behaviors, including cell morphology, adhesion, proliferation, clonal expansion, and self-renewal. Our results indicated that topological sensing of hESCs might include feedback regulation involving mechanosensory integrin-mediated cell-matrix adhesion, myosin II, and E-cadherin. Our results also demonstrated that cellular responses to nanotopography were cell-type specific, and as such, we could generate a spatially segregated coculture system for hESCs and NIH/3T3 fibroblasts using patterned nanorough glass surfaces.

Original languageEnglish (US)
Pages (from-to)4094-4103
Number of pages10
JournalACS Nano
Volume6
Issue number5
DOIs
StatePublished - May 22 2012

Fingerprint

stem cells
Stem cells
adhesion
Adhesion
cells
Myosin Type II
myosins
Glass
glass
Microfabrication
fibroblasts
Cadherins
Fibroblasts
Integrins
Feedback
expansion
matrices

Keywords

  • Human embryonic stem cell
  • Mechanosensitivity
  • Microfabrication
  • Nanotopography
  • Self-renewal

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Chen, W., Villa-Diaz, L. G., Sun, Y., Weng, S., Kim, J. K., Lam, R. H. W., ... Fu, J. (2012). Nanotopography influences adhesion, spreading, and self-renewal of Human embryonic stem cells. ACS Nano, 6(5), 4094-4103. https://doi.org/10.1021/nn3004923

Nanotopography influences adhesion, spreading, and self-renewal of Human embryonic stem cells. / Chen, Weiqiang; Villa-Diaz, Luis G.; Sun, Yubing; Weng, Shinuo; Kim, Jin Koo; Lam, Raymond H W; Han, Lin; Fan, Rong; Krebsbach, Paul H.; Fu, Jianping.

In: ACS Nano, Vol. 6, No. 5, 22.05.2012, p. 4094-4103.

Research output: Contribution to journalArticle

Chen, W, Villa-Diaz, LG, Sun, Y, Weng, S, Kim, JK, Lam, RHW, Han, L, Fan, R, Krebsbach, PH & Fu, J 2012, 'Nanotopography influences adhesion, spreading, and self-renewal of Human embryonic stem cells', ACS Nano, vol. 6, no. 5, pp. 4094-4103. https://doi.org/10.1021/nn3004923
Chen, Weiqiang ; Villa-Diaz, Luis G. ; Sun, Yubing ; Weng, Shinuo ; Kim, Jin Koo ; Lam, Raymond H W ; Han, Lin ; Fan, Rong ; Krebsbach, Paul H. ; Fu, Jianping. / Nanotopography influences adhesion, spreading, and self-renewal of Human embryonic stem cells. In: ACS Nano. 2012 ; Vol. 6, No. 5. pp. 4094-4103.
@article{7cc96f0789694450a3a74eafea4f0b9d,
title = "Nanotopography influences adhesion, spreading, and self-renewal of Human embryonic stem cells",
abstract = "Human embryonic stem cells (hESCs) have great potentials for future cell-based therapeutics. However, their mechanosensitivity to biophysical signals from the cellular microenvironment is not well characterized. Here we introduced an effective microfabrication strategy for accurate control and patterning of nanoroughness on glass surfaces. Our results demonstrated that nanotopography could provide a potent regulatory signal over different hESC behaviors, including cell morphology, adhesion, proliferation, clonal expansion, and self-renewal. Our results indicated that topological sensing of hESCs might include feedback regulation involving mechanosensory integrin-mediated cell-matrix adhesion, myosin II, and E-cadherin. Our results also demonstrated that cellular responses to nanotopography were cell-type specific, and as such, we could generate a spatially segregated coculture system for hESCs and NIH/3T3 fibroblasts using patterned nanorough glass surfaces.",
keywords = "Human embryonic stem cell, Mechanosensitivity, Microfabrication, Nanotopography, Self-renewal",
author = "Weiqiang Chen and Villa-Diaz, {Luis G.} and Yubing Sun and Shinuo Weng and Kim, {Jin Koo} and Lam, {Raymond H W} and Lin Han and Rong Fan and Krebsbach, {Paul H.} and Jianping Fu",
year = "2012",
month = "5",
day = "22",
doi = "10.1021/nn3004923",
language = "English (US)",
volume = "6",
pages = "4094--4103",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "5",

}

TY - JOUR

T1 - Nanotopography influences adhesion, spreading, and self-renewal of Human embryonic stem cells

AU - Chen, Weiqiang

AU - Villa-Diaz, Luis G.

AU - Sun, Yubing

AU - Weng, Shinuo

AU - Kim, Jin Koo

AU - Lam, Raymond H W

AU - Han, Lin

AU - Fan, Rong

AU - Krebsbach, Paul H.

AU - Fu, Jianping

PY - 2012/5/22

Y1 - 2012/5/22

N2 - Human embryonic stem cells (hESCs) have great potentials for future cell-based therapeutics. However, their mechanosensitivity to biophysical signals from the cellular microenvironment is not well characterized. Here we introduced an effective microfabrication strategy for accurate control and patterning of nanoroughness on glass surfaces. Our results demonstrated that nanotopography could provide a potent regulatory signal over different hESC behaviors, including cell morphology, adhesion, proliferation, clonal expansion, and self-renewal. Our results indicated that topological sensing of hESCs might include feedback regulation involving mechanosensory integrin-mediated cell-matrix adhesion, myosin II, and E-cadherin. Our results also demonstrated that cellular responses to nanotopography were cell-type specific, and as such, we could generate a spatially segregated coculture system for hESCs and NIH/3T3 fibroblasts using patterned nanorough glass surfaces.

AB - Human embryonic stem cells (hESCs) have great potentials for future cell-based therapeutics. However, their mechanosensitivity to biophysical signals from the cellular microenvironment is not well characterized. Here we introduced an effective microfabrication strategy for accurate control and patterning of nanoroughness on glass surfaces. Our results demonstrated that nanotopography could provide a potent regulatory signal over different hESC behaviors, including cell morphology, adhesion, proliferation, clonal expansion, and self-renewal. Our results indicated that topological sensing of hESCs might include feedback regulation involving mechanosensory integrin-mediated cell-matrix adhesion, myosin II, and E-cadherin. Our results also demonstrated that cellular responses to nanotopography were cell-type specific, and as such, we could generate a spatially segregated coculture system for hESCs and NIH/3T3 fibroblasts using patterned nanorough glass surfaces.

KW - Human embryonic stem cell

KW - Mechanosensitivity

KW - Microfabrication

KW - Nanotopography

KW - Self-renewal

UR - http://www.scopus.com/inward/record.url?scp=84864661434&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864661434&partnerID=8YFLogxK

U2 - 10.1021/nn3004923

DO - 10.1021/nn3004923

M3 - Article

VL - 6

SP - 4094

EP - 4103

JO - ACS Nano

JF - ACS Nano

SN - 1936-0851

IS - 5

ER -