Nanoscale assembly in biological systems: From neuronal cytoskeletal proteins to curvature stabilizing lipids

Cyrus R. Safinya, Uri Raviv, Daniel J. Needleman, Alexandra Zidovska, Myung Chul Choi, Miguel A. Ojeda-Lopez, Kai K. Ewert, Youli Li, Herbert P. Miller, Joel Quispe, Bridget Carragher, Clinton S. Potter, Mahn Won Kim, Stuart C. Feinstein, Leslie Wilson

    Research output: Contribution to journalArticle

    Abstract

    The review will describe experiments inspired by the rich variety of bundles and networks of interacting microtubules (MT), neurofilaments, and filamentous-actin in neurons where the nature of the interactions, structures, and structure-function correlations remain poorly understood. We describe how three-dimensional (3D) MT bundles and 2D MT bundles may assemble, in cell free systems in the presence of counter-ions, revealing structures not predicted by polyelectrolyte theories. Interestingly, experiments reveal that the neuronal protein tau, an abundant MT-associated-protein in axons, modulates the MT diameter providing insight for the control of geometric parameters in bio- nanotechnology. In another set of experiments we describe lipid-protein- nanotubes, and lipid nano- tubes and rods, resulting from membrane shape evolution processes involving protein templates and curvature stabilizing lipids. Similar membrane shape changes, occurring in cells for the purpose of specific functions, are induced by interactions between membranes and proteins. The biological materials systems described have applications in bio-nanotechnology. Supramolecular structures of bundles and loop-like networks of microtubules in the presence of counterions (blue/red spheres comprise the tubule wall), and nanorods and nanotubes of block liposomes comprised of charged curvature-stabilizing-lipids (green/yellow), determined by synchrotron X-ray scattering and electron microscopy including cryogenic TEM. The distinct microtubule bundles and lipid nanotubes and nanorods have applications in nanotechnology and biotechnology.

    Original languageEnglish (US)
    Pages (from-to)2260-2270
    Number of pages11
    JournalAdvanced Materials
    Volume23
    Issue number20
    DOIs
    StatePublished - May 24 2011

    Fingerprint

    Cytoskeletal Proteins
    Biological systems
    Lipids
    Proteins
    Nanotechnology
    Nanotubes
    Membranes
    Nanorods
    tau Proteins
    Microtubule-Associated Proteins
    Radiation counters
    Liposomes
    Experiments
    Biotechnology
    Polyelectrolytes
    X ray scattering
    Synchrotrons
    Biological materials
    Cryogenics
    Electron microscopy

    Keywords

    • block liposomes
    • Cryo-TEM
    • microtubules
    • neuronal proteins
    • X-ray scattering

    ASJC Scopus subject areas

    • Materials Science(all)
    • Mechanics of Materials
    • Mechanical Engineering

    Cite this

    Safinya, C. R., Raviv, U., Needleman, D. J., Zidovska, A., Choi, M. C., Ojeda-Lopez, M. A., ... Wilson, L. (2011). Nanoscale assembly in biological systems: From neuronal cytoskeletal proteins to curvature stabilizing lipids. Advanced Materials, 23(20), 2260-2270. https://doi.org/10.1002/adma.201004647

    Nanoscale assembly in biological systems : From neuronal cytoskeletal proteins to curvature stabilizing lipids. / Safinya, Cyrus R.; Raviv, Uri; Needleman, Daniel J.; Zidovska, Alexandra; Choi, Myung Chul; Ojeda-Lopez, Miguel A.; Ewert, Kai K.; Li, Youli; Miller, Herbert P.; Quispe, Joel; Carragher, Bridget; Potter, Clinton S.; Kim, Mahn Won; Feinstein, Stuart C.; Wilson, Leslie.

    In: Advanced Materials, Vol. 23, No. 20, 24.05.2011, p. 2260-2270.

    Research output: Contribution to journalArticle

    Safinya, CR, Raviv, U, Needleman, DJ, Zidovska, A, Choi, MC, Ojeda-Lopez, MA, Ewert, KK, Li, Y, Miller, HP, Quispe, J, Carragher, B, Potter, CS, Kim, MW, Feinstein, SC & Wilson, L 2011, 'Nanoscale assembly in biological systems: From neuronal cytoskeletal proteins to curvature stabilizing lipids', Advanced Materials, vol. 23, no. 20, pp. 2260-2270. https://doi.org/10.1002/adma.201004647
    Safinya CR, Raviv U, Needleman DJ, Zidovska A, Choi MC, Ojeda-Lopez MA et al. Nanoscale assembly in biological systems: From neuronal cytoskeletal proteins to curvature stabilizing lipids. Advanced Materials. 2011 May 24;23(20):2260-2270. https://doi.org/10.1002/adma.201004647
    Safinya, Cyrus R. ; Raviv, Uri ; Needleman, Daniel J. ; Zidovska, Alexandra ; Choi, Myung Chul ; Ojeda-Lopez, Miguel A. ; Ewert, Kai K. ; Li, Youli ; Miller, Herbert P. ; Quispe, Joel ; Carragher, Bridget ; Potter, Clinton S. ; Kim, Mahn Won ; Feinstein, Stuart C. ; Wilson, Leslie. / Nanoscale assembly in biological systems : From neuronal cytoskeletal proteins to curvature stabilizing lipids. In: Advanced Materials. 2011 ; Vol. 23, No. 20. pp. 2260-2270.
    @article{e4cd5bdca9ae4d4f992d425ce5b34a49,
    title = "Nanoscale assembly in biological systems: From neuronal cytoskeletal proteins to curvature stabilizing lipids",
    abstract = "The review will describe experiments inspired by the rich variety of bundles and networks of interacting microtubules (MT), neurofilaments, and filamentous-actin in neurons where the nature of the interactions, structures, and structure-function correlations remain poorly understood. We describe how three-dimensional (3D) MT bundles and 2D MT bundles may assemble, in cell free systems in the presence of counter-ions, revealing structures not predicted by polyelectrolyte theories. Interestingly, experiments reveal that the neuronal protein tau, an abundant MT-associated-protein in axons, modulates the MT diameter providing insight for the control of geometric parameters in bio- nanotechnology. In another set of experiments we describe lipid-protein- nanotubes, and lipid nano- tubes and rods, resulting from membrane shape evolution processes involving protein templates and curvature stabilizing lipids. Similar membrane shape changes, occurring in cells for the purpose of specific functions, are induced by interactions between membranes and proteins. The biological materials systems described have applications in bio-nanotechnology. Supramolecular structures of bundles and loop-like networks of microtubules in the presence of counterions (blue/red spheres comprise the tubule wall), and nanorods and nanotubes of block liposomes comprised of charged curvature-stabilizing-lipids (green/yellow), determined by synchrotron X-ray scattering and electron microscopy including cryogenic TEM. The distinct microtubule bundles and lipid nanotubes and nanorods have applications in nanotechnology and biotechnology.",
    keywords = "block liposomes, Cryo-TEM, microtubules, neuronal proteins, X-ray scattering",
    author = "Safinya, {Cyrus R.} and Uri Raviv and Needleman, {Daniel J.} and Alexandra Zidovska and Choi, {Myung Chul} and Ojeda-Lopez, {Miguel A.} and Ewert, {Kai K.} and Youli Li and Miller, {Herbert P.} and Joel Quispe and Bridget Carragher and Potter, {Clinton S.} and Kim, {Mahn Won} and Feinstein, {Stuart C.} and Leslie Wilson",
    year = "2011",
    month = "5",
    day = "24",
    doi = "10.1002/adma.201004647",
    language = "English (US)",
    volume = "23",
    pages = "2260--2270",
    journal = "Advanced Materials",
    issn = "0935-9648",
    publisher = "Wiley-VCH Verlag",
    number = "20",

    }

    TY - JOUR

    T1 - Nanoscale assembly in biological systems

    T2 - From neuronal cytoskeletal proteins to curvature stabilizing lipids

    AU - Safinya, Cyrus R.

    AU - Raviv, Uri

    AU - Needleman, Daniel J.

    AU - Zidovska, Alexandra

    AU - Choi, Myung Chul

    AU - Ojeda-Lopez, Miguel A.

    AU - Ewert, Kai K.

    AU - Li, Youli

    AU - Miller, Herbert P.

    AU - Quispe, Joel

    AU - Carragher, Bridget

    AU - Potter, Clinton S.

    AU - Kim, Mahn Won

    AU - Feinstein, Stuart C.

    AU - Wilson, Leslie

    PY - 2011/5/24

    Y1 - 2011/5/24

    N2 - The review will describe experiments inspired by the rich variety of bundles and networks of interacting microtubules (MT), neurofilaments, and filamentous-actin in neurons where the nature of the interactions, structures, and structure-function correlations remain poorly understood. We describe how three-dimensional (3D) MT bundles and 2D MT bundles may assemble, in cell free systems in the presence of counter-ions, revealing structures not predicted by polyelectrolyte theories. Interestingly, experiments reveal that the neuronal protein tau, an abundant MT-associated-protein in axons, modulates the MT diameter providing insight for the control of geometric parameters in bio- nanotechnology. In another set of experiments we describe lipid-protein- nanotubes, and lipid nano- tubes and rods, resulting from membrane shape evolution processes involving protein templates and curvature stabilizing lipids. Similar membrane shape changes, occurring in cells for the purpose of specific functions, are induced by interactions between membranes and proteins. The biological materials systems described have applications in bio-nanotechnology. Supramolecular structures of bundles and loop-like networks of microtubules in the presence of counterions (blue/red spheres comprise the tubule wall), and nanorods and nanotubes of block liposomes comprised of charged curvature-stabilizing-lipids (green/yellow), determined by synchrotron X-ray scattering and electron microscopy including cryogenic TEM. The distinct microtubule bundles and lipid nanotubes and nanorods have applications in nanotechnology and biotechnology.

    AB - The review will describe experiments inspired by the rich variety of bundles and networks of interacting microtubules (MT), neurofilaments, and filamentous-actin in neurons where the nature of the interactions, structures, and structure-function correlations remain poorly understood. We describe how three-dimensional (3D) MT bundles and 2D MT bundles may assemble, in cell free systems in the presence of counter-ions, revealing structures not predicted by polyelectrolyte theories. Interestingly, experiments reveal that the neuronal protein tau, an abundant MT-associated-protein in axons, modulates the MT diameter providing insight for the control of geometric parameters in bio- nanotechnology. In another set of experiments we describe lipid-protein- nanotubes, and lipid nano- tubes and rods, resulting from membrane shape evolution processes involving protein templates and curvature stabilizing lipids. Similar membrane shape changes, occurring in cells for the purpose of specific functions, are induced by interactions between membranes and proteins. The biological materials systems described have applications in bio-nanotechnology. Supramolecular structures of bundles and loop-like networks of microtubules in the presence of counterions (blue/red spheres comprise the tubule wall), and nanorods and nanotubes of block liposomes comprised of charged curvature-stabilizing-lipids (green/yellow), determined by synchrotron X-ray scattering and electron microscopy including cryogenic TEM. The distinct microtubule bundles and lipid nanotubes and nanorods have applications in nanotechnology and biotechnology.

    KW - block liposomes

    KW - Cryo-TEM

    KW - microtubules

    KW - neuronal proteins

    KW - X-ray scattering

    UR - http://www.scopus.com/inward/record.url?scp=79958822383&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=79958822383&partnerID=8YFLogxK

    U2 - 10.1002/adma.201004647

    DO - 10.1002/adma.201004647

    M3 - Article

    C2 - 21506171

    AN - SCOPUS:79958822383

    VL - 23

    SP - 2260

    EP - 2270

    JO - Advanced Materials

    JF - Advanced Materials

    SN - 0935-9648

    IS - 20

    ER -