Nanometer scale titanium surface texturing are detected by signaling pathways involving transient FAK and Src activations

Willian F. Zambuzzi, Estevam A. Bonfante, Ryo Jimbo, Mariko Hayashi, Martin Andersson, Gutemberg Alves, Esther R. Takamori, Paulo J. Beltrão, Paulo Coelho, José M. Granjeiro

Research output: Contribution to journalArticle

Abstract

Background: It is known that physico/chemical alterations on biomaterial surfaces have the capability to modulate cellular behavior, affecting early tissue repair. Such surface modifications are aimed to improve early healing response and, clinically, offer the possibility to shorten the time from implant placement to functional loading. Since FAK and Src are intracellular proteins able to predict the quality of osteoblast adhesion, this study evaluated the osteoblast behavior in response to nanometer scale titanium surface texturing by monitoring FAK and Src phosphorylations. Methodology: Four engineered titanium surfaces were used for the study: machined (M), dual acid-etched (DAA), resorbable media microblasted and acid-etched (MBAA), and acid-etch microblasted (AAMB). Surfaces were characterized by scanning electron microscopy, interferometry, atomic force microscopy, x-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. Thereafter, those 4 samples were used to evaluate their cytotoxicity and interference on FAK and Src phosphorylations. Both Src and FAK were investigated by using specific antibody against specific phosphorylation sites. Principal Findings: The results showed that both FAK and Src activations were differently modulated as a function of titanium surfaces physico/chemical configuration and protein adsorption. Conclusions: It can be suggested that signaling pathways involving both FAK and Src could provide biomarkers to predict osteoblast adhesion onto different surfaces.

Original languageEnglish (US)
Article numbere95662
JournalPLoS One
Volume9
Issue number7
DOIs
StatePublished - Jul 7 2014

Fingerprint

titanium
Texturing
Titanium
Osteoblasts
Chemical activation
Phosphorylation
Acids
osteoblasts
Interferometry
X-Ray Emission Spectrometry
Photoelectron Spectroscopy
Atomic Force Microscopy
Biocompatible Materials
phosphorylation
Electron Scanning Microscopy
Adsorption
Proteins
Adhesion
Biomarkers
adhesion

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Zambuzzi, W. F., Bonfante, E. A., Jimbo, R., Hayashi, M., Andersson, M., Alves, G., ... Granjeiro, J. M. (2014). Nanometer scale titanium surface texturing are detected by signaling pathways involving transient FAK and Src activations. PLoS One, 9(7), [e95662]. https://doi.org/10.1371/journal.pone.0095662

Nanometer scale titanium surface texturing are detected by signaling pathways involving transient FAK and Src activations. / Zambuzzi, Willian F.; Bonfante, Estevam A.; Jimbo, Ryo; Hayashi, Mariko; Andersson, Martin; Alves, Gutemberg; Takamori, Esther R.; Beltrão, Paulo J.; Coelho, Paulo; Granjeiro, José M.

In: PLoS One, Vol. 9, No. 7, e95662, 07.07.2014.

Research output: Contribution to journalArticle

Zambuzzi, WF, Bonfante, EA, Jimbo, R, Hayashi, M, Andersson, M, Alves, G, Takamori, ER, Beltrão, PJ, Coelho, P & Granjeiro, JM 2014, 'Nanometer scale titanium surface texturing are detected by signaling pathways involving transient FAK and Src activations', PLoS One, vol. 9, no. 7, e95662. https://doi.org/10.1371/journal.pone.0095662
Zambuzzi, Willian F. ; Bonfante, Estevam A. ; Jimbo, Ryo ; Hayashi, Mariko ; Andersson, Martin ; Alves, Gutemberg ; Takamori, Esther R. ; Beltrão, Paulo J. ; Coelho, Paulo ; Granjeiro, José M. / Nanometer scale titanium surface texturing are detected by signaling pathways involving transient FAK and Src activations. In: PLoS One. 2014 ; Vol. 9, No. 7.
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