Nanofluidic preconcentration device in a straight microchannel using ion concentration polarization

Sung Hee Ko, Rafael Song, Sung Jae Kim, Myungji Kim, Jongyoon Han, Kwan Hyoung Kang

    Research output: Contribution to journalArticle

    Abstract

    In this paper, we introduce a simple, straight microchannel design for a nanofluidic protein concentration device. Compared with concentration devices previously developed, the anode channel and cathode channel in this new concentration scheme are both integrated into a straight microchannel, with one inlet and one outlet. Most of the functions of a conventional two-channel concentration device can be achieved by this concentration device, and the efficiency of sample accumulation can be controlled by the dimension of the Nafion membrane. Also, the operating mechanism of this device was tested on various material combinations such as PDMS (polydimethyl-siloxane) channel-glass substrate and silicon channel-PDMS substrate. Using a combined PDMS-silicon device which was sealed reversibly without plasma bonding, surface based immunoassay for concentrator-enhanced detection of clinically relevant samples such as C-reactive protein (CRP) was demonstrated. As a result, it was possible to enhance the detection sensitivity of the immunoassay by more than 500 folds compared to the immunoassay without preconcentration process.

    Original languageEnglish (US)
    Pages (from-to)4472-4482
    Number of pages11
    JournalLab on a Chip
    Volume12
    Issue number21
    DOIs
    StatePublished - Nov 7 2012

    Fingerprint

    Nanofluidics
    Siloxanes
    Microchannels
    Silicon
    Ions
    Polarization
    Proteins
    Equipment and Supplies
    Substrates
    Immunoassay
    Anodes
    Cathodes
    Membranes
    Plasmas
    Glass
    C-Reactive Protein
    Electrodes

    ASJC Scopus subject areas

    • Bioengineering
    • Biochemistry
    • Chemistry(all)
    • Biomedical Engineering

    Cite this

    Nanofluidic preconcentration device in a straight microchannel using ion concentration polarization. / Ko, Sung Hee; Song, Rafael; Kim, Sung Jae; Kim, Myungji; Han, Jongyoon; Kang, Kwan Hyoung.

    In: Lab on a Chip, Vol. 12, No. 21, 07.11.2012, p. 4472-4482.

    Research output: Contribution to journalArticle

    Ko, Sung Hee ; Song, Rafael ; Kim, Sung Jae ; Kim, Myungji ; Han, Jongyoon ; Kang, Kwan Hyoung. / Nanofluidic preconcentration device in a straight microchannel using ion concentration polarization. In: Lab on a Chip. 2012 ; Vol. 12, No. 21. pp. 4472-4482.
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