NADPH oxidase mediates -amyloid peptide-induced activation of ERK in hippocampal organotypic cultures

Faridis Serrano, Angela Chang, Caterina Hernandez, Robia G. Pautler, J. David Sweatt, Eric Klann

Research output: Contribution to journalArticle

Abstract

Background: Previous studies have shown that beta amyloid (A) peptide triggers the activation of several signal transduction cascades in the hippocampus, including the extracellular signal-regulated kinase (ERK) cascade. In this study we sought to characterize the cellular localization of phosphorylated, active ERK in organotypic hippocampal cultures after acute exposure to either A (1-42) or nicotine. Results. We observed that A and nicotine increased the levels of active ERK in distinct cellular localizations. We also examined whether phospho-ERK was regulated by redox signaling mechanisms and found that increases in active ERK induced by A and nicotine were blocked by inhibitors of NADPH oxidase. Conclusion. Our findings indicate that NADPH oxidase-dependent redox signaling is required for A-induced activation of ERK, and suggest a similar mechanism may occur during early stages of Alzheimer's disease.

Original languageEnglish (US)
Article number31
JournalMolecular Brain
Volume2
Issue number1
DOIs
StatePublished - 2009

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NADPH Oxidase
Extracellular Signal-Regulated MAP Kinases
Amyloid
Peptides
Nicotine
Oxidation-Reduction
Amyloid beta-Peptides
Signal Transduction
Hippocampus
Alzheimer Disease

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Molecular Biology

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NADPH oxidase mediates -amyloid peptide-induced activation of ERK in hippocampal organotypic cultures. / Serrano, Faridis; Chang, Angela; Hernandez, Caterina; Pautler, Robia G.; Sweatt, J. David; Klann, Eric.

In: Molecular Brain, Vol. 2, No. 1, 31, 2009.

Research output: Contribution to journalArticle

Serrano, Faridis ; Chang, Angela ; Hernandez, Caterina ; Pautler, Robia G. ; Sweatt, J. David ; Klann, Eric. / NADPH oxidase mediates -amyloid peptide-induced activation of ERK in hippocampal organotypic cultures. In: Molecular Brain. 2009 ; Vol. 2, No. 1.
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