Nacre protein fragment templates lamellar aragonite growth

Rebecca A. Metzler, John Evans, Christopher E. Killian, Dong Zhou, Tyler H. Churchill, Narayana P. Appathurai, Susan N. Coppersmith, P. U P A Gilbert

Research output: Contribution to journalArticle

Abstract

Proteins play a major role in the formation of all biominerals. In mollusk shell nacre, complex mixtures and assemblies of proteins and polysaccharides were shown to induce aragonite formation, rather than the thermodynamically favored calcite (both aragonite and calcite are CaCO3 polymorphs). Here we used N16N, a single 30 amino acid-protein fragment originally inspired by the mineral binding site of N16, a protein in the nacre layer of the Japanese pearl oysters (Pinctada fucata). In a calcite growth solution this short peptide induces in vitro biomineralization. This model biomineral was analyzed using X-ray PhotoElectron Emission spectroMicroscopy (X-PEEM) and found to be strikingly similar to natural nacre: lamellar aragonite with interspersed N16N layers. This and other findings combined suggest a hypothetical scenario in which in vivo three proteins (N16, Pif80, and Pif97) and a polysaccharide (chitin) work in concert to form lamellar nacre.

Original languageEnglish (US)
Pages (from-to)6329-6334
Number of pages6
JournalJournal of the American Chemical Society
Volume132
Issue number18
DOIs
StatePublished - May 12 2010

Fingerprint

Nacre
Calcium Carbonate
Proteins
Calcite
Pinctada
Growth
Polysaccharides
Biomineralization
Chitin
Binding sites
Photoelectrons
Polymorphism
Mollusca
Peptides
Amino acids
Complex Mixtures
Minerals
X rays
Binding Sites
X-Rays

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Metzler, R. A., Evans, J., Killian, C. E., Zhou, D., Churchill, T. H., Appathurai, N. P., ... Gilbert, P. U. P. A. (2010). Nacre protein fragment templates lamellar aragonite growth. Journal of the American Chemical Society, 132(18), 6329-6334. https://doi.org/10.1021/ja909735y

Nacre protein fragment templates lamellar aragonite growth. / Metzler, Rebecca A.; Evans, John; Killian, Christopher E.; Zhou, Dong; Churchill, Tyler H.; Appathurai, Narayana P.; Coppersmith, Susan N.; Gilbert, P. U P A.

In: Journal of the American Chemical Society, Vol. 132, No. 18, 12.05.2010, p. 6329-6334.

Research output: Contribution to journalArticle

Metzler, RA, Evans, J, Killian, CE, Zhou, D, Churchill, TH, Appathurai, NP, Coppersmith, SN & Gilbert, PUPA 2010, 'Nacre protein fragment templates lamellar aragonite growth', Journal of the American Chemical Society, vol. 132, no. 18, pp. 6329-6334. https://doi.org/10.1021/ja909735y
Metzler RA, Evans J, Killian CE, Zhou D, Churchill TH, Appathurai NP et al. Nacre protein fragment templates lamellar aragonite growth. Journal of the American Chemical Society. 2010 May 12;132(18):6329-6334. https://doi.org/10.1021/ja909735y
Metzler, Rebecca A. ; Evans, John ; Killian, Christopher E. ; Zhou, Dong ; Churchill, Tyler H. ; Appathurai, Narayana P. ; Coppersmith, Susan N. ; Gilbert, P. U P A. / Nacre protein fragment templates lamellar aragonite growth. In: Journal of the American Chemical Society. 2010 ; Vol. 132, No. 18. pp. 6329-6334.
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