N2 fixation as a dominant new N source in the western tropical South Pacific Ocean (OUTPACE cruise)

Mathieu Caffin, Thierry Moutin, Rachel Ann Foster, Pascale Bouruet-Aubertot, Andrea Michelangelo Doglioli, Hugo Berthelot, Cecile Guieu, Olivier Grosso, Sandra Helias-Nunige, Nathalie Leblond, Audrey Gimenez, Anne Alexandra Petrenko, Alain De Verneil, Sophie Bonnet

Research output: Contribution to journalArticle

Abstract

We performed nitrogen (N) budgets in the photic layer of three contrasting stations representing different trophic conditions in the western tropical South Pacific (WTSP) Ocean during austral summer conditions (February-March 2015). Using a Lagrangian strategy, we sampled the same water mass for the entire duration of each long-duration (5 days) station, allowing us to consider only vertical exchanges for the budgets. We quantified all major vertical N fluxes both entering (N2 fixation, nitrate turbulent diffusion, atmospheric deposition) and leaving the photic layer (particulate N export). The three stations were characterized by a strong nitracline and contrasted deep chlorophyll maximum depths, which were lower in the oligotrophic Melanesian archipelago (MA, stations LD A and LD B) than in the ultra-oligotrophic waters of the South Pacific Gyre (SPG, station LD C). N2fixation rates were extremely high at both LD A (593g±51gμmol N m2d1) and LD B (706g±302 N m2d1) and the diazotroph community was dominated by Trichodesmium N2 fixation rates were lower (59g±16μmol N m2d1 at LD C, and the diazotroph community was dominated by unicellular N2-fixing cyanobacteria (UCYN). At all stations, N2 fixation was the major source of new N > 90%) before atmospheric deposition and upward nitrate fluxes induced by turbulence. N2 fixation contributed circa 13-18% of primary production in the MA region and 3% in the SPG water and sustained nearly all new primary production at all stations. The e ratio (e ratiog = particulate carbon exportg/primary production) was maximum at LD A (9.7%) and was higher than the e ratio in most studied oligotrophic regions (<5%), indicating a high efficiency of the WTSP to export carbon relative to primary production. The direct export of diazotrophs assessed by qPCR of the nifH gene in sediment traps represented up to 30.6% of the PC export at LD A, while their contribution was 5 and < 0.1% at LD B and LD C, respectively. At the three studied stations, the sum of all N input to the photic layer exceeded the N output through organic matter export. This disequilibrium leading to N accumulation in the upper layer appears as a characteristic of the WTSP during the summer season.

Original languageEnglish (US)
Pages (from-to)2565-2585
Number of pages21
JournalBiogeosciences
Volume15
Issue number8
DOIs
StatePublished - May 2 2018

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Pacific Ocean
fixation
primary productivity
atmospheric deposition
primary production
particulates
nitrates
Trichodesmium
nitrogen-fixing bacteria
duration
carbon
summer
Cyanobacteria
nitrate
trophic conditions
water
turbulent diffusion
station
South Pacific Ocean
organic matter

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Earth-Surface Processes

Cite this

Caffin, M., Moutin, T., Ann Foster, R., Bouruet-Aubertot, P., Michelangelo Doglioli, A., Berthelot, H., ... Bonnet, S. (2018). N2 fixation as a dominant new N source in the western tropical South Pacific Ocean (OUTPACE cruise). Biogeosciences, 15(8), 2565-2585. https://doi.org/10.5194/bg-15-2565-2018

N2 fixation as a dominant new N source in the western tropical South Pacific Ocean (OUTPACE cruise). / Caffin, Mathieu; Moutin, Thierry; Ann Foster, Rachel; Bouruet-Aubertot, Pascale; Michelangelo Doglioli, Andrea; Berthelot, Hugo; Guieu, Cecile; Grosso, Olivier; Helias-Nunige, Sandra; Leblond, Nathalie; Gimenez, Audrey; Alexandra Petrenko, Anne; De Verneil, Alain; Bonnet, Sophie.

In: Biogeosciences, Vol. 15, No. 8, 02.05.2018, p. 2565-2585.

Research output: Contribution to journalArticle

Caffin, M, Moutin, T, Ann Foster, R, Bouruet-Aubertot, P, Michelangelo Doglioli, A, Berthelot, H, Guieu, C, Grosso, O, Helias-Nunige, S, Leblond, N, Gimenez, A, Alexandra Petrenko, A, De Verneil, A & Bonnet, S 2018, 'N2 fixation as a dominant new N source in the western tropical South Pacific Ocean (OUTPACE cruise)', Biogeosciences, vol. 15, no. 8, pp. 2565-2585. https://doi.org/10.5194/bg-15-2565-2018
Caffin M, Moutin T, Ann Foster R, Bouruet-Aubertot P, Michelangelo Doglioli A, Berthelot H et al. N2 fixation as a dominant new N source in the western tropical South Pacific Ocean (OUTPACE cruise). Biogeosciences. 2018 May 2;15(8):2565-2585. https://doi.org/10.5194/bg-15-2565-2018
Caffin, Mathieu ; Moutin, Thierry ; Ann Foster, Rachel ; Bouruet-Aubertot, Pascale ; Michelangelo Doglioli, Andrea ; Berthelot, Hugo ; Guieu, Cecile ; Grosso, Olivier ; Helias-Nunige, Sandra ; Leblond, Nathalie ; Gimenez, Audrey ; Alexandra Petrenko, Anne ; De Verneil, Alain ; Bonnet, Sophie. / N2 fixation as a dominant new N source in the western tropical South Pacific Ocean (OUTPACE cruise). In: Biogeosciences. 2018 ; Vol. 15, No. 8. pp. 2565-2585.
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AU - Caffin, Mathieu

AU - Moutin, Thierry

AU - Ann Foster, Rachel

AU - Bouruet-Aubertot, Pascale

AU - Michelangelo Doglioli, Andrea

AU - Berthelot, Hugo

AU - Guieu, Cecile

AU - Grosso, Olivier

AU - Helias-Nunige, Sandra

AU - Leblond, Nathalie

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AU - Alexandra Petrenko, Anne

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N2 - We performed nitrogen (N) budgets in the photic layer of three contrasting stations representing different trophic conditions in the western tropical South Pacific (WTSP) Ocean during austral summer conditions (February-March 2015). Using a Lagrangian strategy, we sampled the same water mass for the entire duration of each long-duration (5 days) station, allowing us to consider only vertical exchanges for the budgets. We quantified all major vertical N fluxes both entering (N2 fixation, nitrate turbulent diffusion, atmospheric deposition) and leaving the photic layer (particulate N export). The three stations were characterized by a strong nitracline and contrasted deep chlorophyll maximum depths, which were lower in the oligotrophic Melanesian archipelago (MA, stations LD A and LD B) than in the ultra-oligotrophic waters of the South Pacific Gyre (SPG, station LD C). N2fixation rates were extremely high at both LD A (593g±51gμmol N m2d1) and LD B (706g±302 N m2d1) and the diazotroph community was dominated by Trichodesmium N2 fixation rates were lower (59g±16μmol N m2d1 at LD C, and the diazotroph community was dominated by unicellular N2-fixing cyanobacteria (UCYN). At all stations, N2 fixation was the major source of new N > 90%) before atmospheric deposition and upward nitrate fluxes induced by turbulence. N2 fixation contributed circa 13-18% of primary production in the MA region and 3% in the SPG water and sustained nearly all new primary production at all stations. The e ratio (e ratiog = particulate carbon exportg/primary production) was maximum at LD A (9.7%) and was higher than the e ratio in most studied oligotrophic regions (<5%), indicating a high efficiency of the WTSP to export carbon relative to primary production. The direct export of diazotrophs assessed by qPCR of the nifH gene in sediment traps represented up to 30.6% of the PC export at LD A, while their contribution was 5 and < 0.1% at LD B and LD C, respectively. At the three studied stations, the sum of all N input to the photic layer exceeded the N output through organic matter export. This disequilibrium leading to N accumulation in the upper layer appears as a characteristic of the WTSP during the summer season.

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