Myofibrillar and cytoskeletal assembly in neonatal rat cardiac myocytes cultured on laminin and collagen

Lula L. Hilenski, Louis Terracio, Thomas K. Borg

Research output: Contribution to journalArticle

Abstract

Neonatal rat cardiomyocytes were cultured on extracellular matrix components laminin and collagens I+III to examine effects of extracellular matrix on the assembly of cytoskeletal proteins during myofibrillogenesis. Myofibril assembly was visualized by immunofluorescence of marker proteins for myofibrils (f-actin for I bands and α-actinin for Z bands), focal adhesions (vinculin), and transmembrane extracellular matrix receptors (β1 integrin) as cells spread for various times in culture. By 4 h in culture, f-actin appeared organized into nonstriated stress-fiber-like structures while α-actinin, vinculin and β1 integrin were localized in small streaks and beads. Subsequently, striated patterns were observed sequentially in the intracellular cytoskeletal components α-actinin, vinculin, f-actin, and then in the transmembrane β1 integrin receptor. These data support an earlier model for sarcomerogenesis in which stress-fiber-like structures serve as initial scaffolds upon which α-actinin and then vinculin-containing costameres are assembled. This sequential and temporal assembly was the same on both laminin and collagens I+III. A quantitative difference, however, was apparent on the 2 matrices. There was an increased appearance on collagens I+III of rosettes (also called podosomes or cortical actin-containing bodies in other cells) which consisted of an f-actin core surrounded by α-actinin, vinculin and β1 integrin rims. The increased incidence of rosettes in neonatal myocytes on collagens I+III suggests that these cytoskeletal complexes are involved in recognition and interaction with extracellular matrix components.

Original languageEnglish (US)
Pages (from-to)577-587
Number of pages11
JournalCell and Tissue Research
Volume264
Issue number3
DOIs
StatePublished - Jun 1991

Fingerprint

Vinculin
Actinin
Laminin
Cardiac Myocytes
Rats
Actins
Collagen
Integrins
Extracellular Matrix
Stress Fibers
Myofibrils
Costameres
Focal Adhesions
Cytoskeletal Proteins
Muscle Development
Fibers
Scaffolds
Muscle Cells
Fluorescent Antibody Technique
Adhesion

Keywords

  • Actin filaments
  • Collage
  • Cytoskeleton
  • Extracellular matrix
  • Laminin
  • Myofibrils
  • Vinculin-Rat

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Myofibrillar and cytoskeletal assembly in neonatal rat cardiac myocytes cultured on laminin and collagen. / Hilenski, Lula L.; Terracio, Louis; Borg, Thomas K.

In: Cell and Tissue Research, Vol. 264, No. 3, 06.1991, p. 577-587.

Research output: Contribution to journalArticle

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