Mutagenesis by N-nitroso compounds in Salmonella typhimurium TA102 and TA104: Evidence for premutagenic adenine or thymine DNA adducts

Joseph Guttenplan, You Chiu Hu

Research output: Contribution to journalArticle

Abstract

Mutagenesis induced by the N-nitroso compounds: N-nitrosomethylurea, N-nitrosoethylurea, N-nitrosodi-n-propylamine and N-nitrosopyrrolidine was measured in Salmonella typhimurium TA100, TA102 and TA104. TA100 detects damage mainly at G-C base pairs while TA102 and TA104 can detect damage at A-T base pairs. In general all strains were similarly sensitive, except that TA104 was much less sensitive to high doses of N-nitroso-N-methylurea. In TA104 a significant percentage of the revertants induced by all agents except NMU resulted from point mutations at A-T base pairs, indicating that adenine or thymine DNA adducts are important premutagenic adducts formed by certain N-nitroso compounds.

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalMutation Research Letters
Volume141
Issue number3-4
DOIs
StatePublished - 1984

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Nitroso Compounds
Mutagenesis
Salmonella
Thymine
DNA Adducts
Adenine
Salmonella typhimurium
Base Pairing
N-Nitrosopyrrolidine
Propylamines
Ethylnitrosourea
Methylnitrosourea
Point Mutation
methylurea

ASJC Scopus subject areas

  • Genetics
  • Toxicology
  • Medicine(all)

Cite this

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abstract = "Mutagenesis induced by the N-nitroso compounds: N-nitrosomethylurea, N-nitrosoethylurea, N-nitrosodi-n-propylamine and N-nitrosopyrrolidine was measured in Salmonella typhimurium TA100, TA102 and TA104. TA100 detects damage mainly at G-C base pairs while TA102 and TA104 can detect damage at A-T base pairs. In general all strains were similarly sensitive, except that TA104 was much less sensitive to high doses of N-nitroso-N-methylurea. In TA104 a significant percentage of the revertants induced by all agents except NMU resulted from point mutations at A-T base pairs, indicating that adenine or thymine DNA adducts are important premutagenic adducts formed by certain N-nitroso compounds.",
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AU - Hu, You Chiu

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