Muscarinic acetylcholine receptors in macaque V1 are most frequently expressed by parvalbumin-immunoreactive neurons

Anita A. Disney, Chiye Aoki

Research output: Contribution to journalArticle

Abstract

Acetylcholine (ACh) is believed to underlie mechanisms of arousal and attention in mammals. ACh also has a demonstrated functional effect in visual cortex that is both diverse and profound. We have reported previously that cholinergic modulation in V1 of the macaque monkey is strongly targeted toward GABAergic interneurons. Here we examine the localization of m1 and m2 muscarinic receptor subtypes across subpopulations of GABAergic interneurons - identified by their expression of the calcium-binding proteins parvalbumin, calbindin, and calretinin - using dual-immunofluorescence confocal microscopy in V1 of the macaque monkey. In doing so, we find that the vast majority (87%) of parvalbumin-immunoreactive neurons express m1-type muscarinic ACh receptors. m1 receptors are also expressed by 60% of calbindin-immunoreactive neurons and 40% of calretinin-immunoreactive neurons. m2 AChRs, on the other hand, are expressed by only 31% of parvalbumin neurons, 23% of calbindin neurons, and 25% of calretinin neurons. Parvalbumin-immunoreactive cells comprise ≈75% of the inhibitory neuronal population in V1 and included in this large subpopulation are neurons known to veto and regulate the synchrony of principal cell spiking. Through the expression of m1 ACh receptors on nearly all of these PV cells, the cholinergic system avails itself of powerful control of information flow through and processing within the network of principal cells in the cortical circuit.

Original languageEnglish (US)
Pages (from-to)1748-1762
Number of pages15
JournalJournal of Comparative Neurology
Volume507
Issue number5
DOIs
StatePublished - Apr 10 2008

Fingerprint

Parvalbumins
Macaca
Muscarinic Receptors
Neurons
Calbindin 2
Calbindins
Interneurons
Cholinergic Agents
Acetylcholine
Haplorhini
Muscarinic M2 Receptors
Muscarinic M1 Receptors
Calcium-Binding Proteins
Cholinergic Receptors
Visual Cortex
Arousal
Fluorescence Microscopy
Confocal Microscopy
Mammals
Population

Keywords

  • Calbindin
  • Calcium-binding proteins
  • Calretinin
  • Cholinergic
  • Dual-labeling
  • GABAergic
  • Immunofluorescence
  • Neuromodulation
  • Parvalbumin
  • Striate cortex

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Muscarinic acetylcholine receptors in macaque V1 are most frequently expressed by parvalbumin-immunoreactive neurons. / Disney, Anita A.; Aoki, Chiye.

In: Journal of Comparative Neurology, Vol. 507, No. 5, 10.04.2008, p. 1748-1762.

Research output: Contribution to journalArticle

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abstract = "Acetylcholine (ACh) is believed to underlie mechanisms of arousal and attention in mammals. ACh also has a demonstrated functional effect in visual cortex that is both diverse and profound. We have reported previously that cholinergic modulation in V1 of the macaque monkey is strongly targeted toward GABAergic interneurons. Here we examine the localization of m1 and m2 muscarinic receptor subtypes across subpopulations of GABAergic interneurons - identified by their expression of the calcium-binding proteins parvalbumin, calbindin, and calretinin - using dual-immunofluorescence confocal microscopy in V1 of the macaque monkey. In doing so, we find that the vast majority (87{\%}) of parvalbumin-immunoreactive neurons express m1-type muscarinic ACh receptors. m1 receptors are also expressed by 60{\%} of calbindin-immunoreactive neurons and 40{\%} of calretinin-immunoreactive neurons. m2 AChRs, on the other hand, are expressed by only 31{\%} of parvalbumin neurons, 23{\%} of calbindin neurons, and 25{\%} of calretinin neurons. Parvalbumin-immunoreactive cells comprise ≈75{\%} of the inhibitory neuronal population in V1 and included in this large subpopulation are neurons known to veto and regulate the synchrony of principal cell spiking. Through the expression of m1 ACh receptors on nearly all of these PV cells, the cholinergic system avails itself of powerful control of information flow through and processing within the network of principal cells in the cortical circuit.",
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AB - Acetylcholine (ACh) is believed to underlie mechanisms of arousal and attention in mammals. ACh also has a demonstrated functional effect in visual cortex that is both diverse and profound. We have reported previously that cholinergic modulation in V1 of the macaque monkey is strongly targeted toward GABAergic interneurons. Here we examine the localization of m1 and m2 muscarinic receptor subtypes across subpopulations of GABAergic interneurons - identified by their expression of the calcium-binding proteins parvalbumin, calbindin, and calretinin - using dual-immunofluorescence confocal microscopy in V1 of the macaque monkey. In doing so, we find that the vast majority (87%) of parvalbumin-immunoreactive neurons express m1-type muscarinic ACh receptors. m1 receptors are also expressed by 60% of calbindin-immunoreactive neurons and 40% of calretinin-immunoreactive neurons. m2 AChRs, on the other hand, are expressed by only 31% of parvalbumin neurons, 23% of calbindin neurons, and 25% of calretinin neurons. Parvalbumin-immunoreactive cells comprise ≈75% of the inhibitory neuronal population in V1 and included in this large subpopulation are neurons known to veto and regulate the synchrony of principal cell spiking. Through the expression of m1 ACh receptors on nearly all of these PV cells, the cholinergic system avails itself of powerful control of information flow through and processing within the network of principal cells in the cortical circuit.

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