Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane

Jeong Hoon Lee, Rafael Song, Jongyoon Han

Research output: Contribution to journalArticle

Abstract

In this paper, we report a new method of fabricating a high-throughput protein preconcentrator in poly(dimethylsiloxane) (PDMS) microfluidic chip format. We print a submicron thick ion-selective membrane on the glass substrate by using standard patterning techniques. By simply plasma-bonding a PDMS microfluidic device on top of the printed glass substrate, we can integrate the ion-selective membrane into the device and rapidly prototype a PDMS preconcentrator without complicated microfabrication and cumbersome integration processes. The PDMS preconcentrator shows a concentration factor as high as ∼104 in 5 min. This printing method even allows fabricating a parallel array of preconcentrators to increase the concentrated sample volume, which can facilitate an integration of our microfluidic preconcentrator chip as a signal enhancing tool to various detectors such as a mass spectrometer.

Original languageEnglish (US)
Pages (from-to)596-601
Number of pages6
JournalLab on a Chip
Volume8
Issue number4
DOIs
StatePublished - Jan 1 2008

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Ion selective membranes
Polydimethylsiloxane
Proteomics
Microfluidics
Ions
Equipment and Supplies
Membranes
Lab-On-A-Chip Devices
Glass
Microtechnology
Printing
Microfabrication
Mass spectrometers
Substrates
Throughput
Detectors
Proteins
Plasmas
baysilon

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane. / Lee, Jeong Hoon; Song, Rafael; Han, Jongyoon.

In: Lab on a Chip, Vol. 8, No. 4, 01.01.2008, p. 596-601.

Research output: Contribution to journalArticle

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