Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane

Jeong Hoon Lee, Rafael Song, Jongyoon Han

    Research output: Contribution to journalArticle

    Abstract

    In this paper, we report a new method of fabricating a high-throughput protein preconcentrator in poly(dimethylsiloxane) (PDMS) microfluidic chip format. We print a submicron thick ion-selective membrane on the glass substrate by using standard patterning techniques. By simply plasma-bonding a PDMS microfluidic device on top of the printed glass substrate, we can integrate the ion-selective membrane into the device and rapidly prototype a PDMS preconcentrator without complicated microfabrication and cumbersome integration processes. The PDMS preconcentrator shows a concentration factor as high as ∼104 in 5 min. This printing method even allows fabricating a parallel array of preconcentrators to increase the concentrated sample volume, which can facilitate an integration of our microfluidic preconcentrator chip as a signal enhancing tool to various detectors such as a mass spectrometer.

    Original languageEnglish (US)
    Pages (from-to)596-601
    Number of pages6
    JournalLab on a Chip
    Volume8
    Issue number4
    DOIs
    StatePublished - Jan 1 2008

    Fingerprint

    Ion selective membranes
    Polydimethylsiloxane
    Proteomics
    Microfluidics
    Ions
    Equipment and Supplies
    Membranes
    Lab-On-A-Chip Devices
    Glass
    Microtechnology
    Printing
    Microfabrication
    Mass spectrometers
    Substrates
    Throughput
    Detectors
    Proteins
    Plasmas
    baysilon

    ASJC Scopus subject areas

    • Bioengineering
    • Biochemistry
    • Chemistry(all)
    • Biomedical Engineering

    Cite this

    Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane. / Lee, Jeong Hoon; Song, Rafael; Han, Jongyoon.

    In: Lab on a Chip, Vol. 8, No. 4, 01.01.2008, p. 596-601.

    Research output: Contribution to journalArticle

    Lee, Jeong Hoon ; Song, Rafael ; Han, Jongyoon. / Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane. In: Lab on a Chip. 2008 ; Vol. 8, No. 4. pp. 596-601.
    @article{a308f0a6ffa94041932d8247283bb6c3,
    title = "Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane",
    abstract = "In this paper, we report a new method of fabricating a high-throughput protein preconcentrator in poly(dimethylsiloxane) (PDMS) microfluidic chip format. We print a submicron thick ion-selective membrane on the glass substrate by using standard patterning techniques. By simply plasma-bonding a PDMS microfluidic device on top of the printed glass substrate, we can integrate the ion-selective membrane into the device and rapidly prototype a PDMS preconcentrator without complicated microfabrication and cumbersome integration processes. The PDMS preconcentrator shows a concentration factor as high as ∼104 in 5 min. This printing method even allows fabricating a parallel array of preconcentrators to increase the concentrated sample volume, which can facilitate an integration of our microfluidic preconcentrator chip as a signal enhancing tool to various detectors such as a mass spectrometer.",
    author = "Lee, {Jeong Hoon} and Rafael Song and Jongyoon Han",
    year = "2008",
    month = "1",
    day = "1",
    doi = "10.1039/b717900f",
    language = "English (US)",
    volume = "8",
    pages = "596--601",
    journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",
    issn = "1473-0197",
    publisher = "Royal Society of Chemistry",
    number = "4",

    }

    TY - JOUR

    T1 - Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane

    AU - Lee, Jeong Hoon

    AU - Song, Rafael

    AU - Han, Jongyoon

    PY - 2008/1/1

    Y1 - 2008/1/1

    N2 - In this paper, we report a new method of fabricating a high-throughput protein preconcentrator in poly(dimethylsiloxane) (PDMS) microfluidic chip format. We print a submicron thick ion-selective membrane on the glass substrate by using standard patterning techniques. By simply plasma-bonding a PDMS microfluidic device on top of the printed glass substrate, we can integrate the ion-selective membrane into the device and rapidly prototype a PDMS preconcentrator without complicated microfabrication and cumbersome integration processes. The PDMS preconcentrator shows a concentration factor as high as ∼104 in 5 min. This printing method even allows fabricating a parallel array of preconcentrators to increase the concentrated sample volume, which can facilitate an integration of our microfluidic preconcentrator chip as a signal enhancing tool to various detectors such as a mass spectrometer.

    AB - In this paper, we report a new method of fabricating a high-throughput protein preconcentrator in poly(dimethylsiloxane) (PDMS) microfluidic chip format. We print a submicron thick ion-selective membrane on the glass substrate by using standard patterning techniques. By simply plasma-bonding a PDMS microfluidic device on top of the printed glass substrate, we can integrate the ion-selective membrane into the device and rapidly prototype a PDMS preconcentrator without complicated microfabrication and cumbersome integration processes. The PDMS preconcentrator shows a concentration factor as high as ∼104 in 5 min. This printing method even allows fabricating a parallel array of preconcentrators to increase the concentrated sample volume, which can facilitate an integration of our microfluidic preconcentrator chip as a signal enhancing tool to various detectors such as a mass spectrometer.

    UR - http://www.scopus.com/inward/record.url?scp=41149176123&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=41149176123&partnerID=8YFLogxK

    U2 - 10.1039/b717900f

    DO - 10.1039/b717900f

    M3 - Article

    VL - 8

    SP - 596

    EP - 601

    JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

    JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

    SN - 1473-0197

    IS - 4

    ER -