Multicolour fluorescenced in situ hybridisation to order small, single-copy probes on metaphase chromosomes

A. C. Heppell-Parton, Donna Albertson, R. Fishpool, P. H. Rabbitts

Research output: Contribution to journalArticle

Abstract

In constructing complete human chromosome maps, the relative order of markers and their precise chromosomal location will be combined. Multicolour in situ hybridisation, in which two probes are simultaneously hybridised to chromosomes and subsequently distinguished, potentially will provide both types of information. Using this technique, we have produced an ordered map of eight human chromosome 3 DNA markers, using small, single-copy probes that can detect target sequences ranging in size from 4 kb to as little as 500 bp.

Original languageEnglish (US)
Pages (from-to)42-47
Number of pages6
JournalCytogenetics and Cell Genetics
Volume66
Issue number1
StatePublished - 1994

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Human Chromosomes
Metaphase
In Situ Hybridization
Chromosomes
Chromosomes, Human, Pair 3
Genetic Markers

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Multicolour fluorescenced in situ hybridisation to order small, single-copy probes on metaphase chromosomes. / Heppell-Parton, A. C.; Albertson, Donna; Fishpool, R.; Rabbitts, P. H.

In: Cytogenetics and Cell Genetics, Vol. 66, No. 1, 1994, p. 42-47.

Research output: Contribution to journalArticle

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