mRNA capping enzyme requirement for Caenorhabditis elegans viability

Priya Srinivasan, Fabio Piano, Aaron J. Shatkin

Research output: Contribution to journalArticle

Abstract

Capping of the initiated 5′ ends of RNA polymerase II products is evolutionarily and functionally conserved from yeasts to humans. The m 7GpppN cap promotes RNA stability, processing, transport, and translation. Deletion of capping enzymes in yeasts was shown to be lethal due to rapid exonucleolytic degradation of uncapped transcripts or failure of capped but unmethylated RNA to initiate protein synthesis. Using RNA interference and Caenorhabditis elegans we have found that RNA capping is also essential for metazoan viability. C. elegans bifunctional capping enzyme was cloned, and capping activity by the expressed protein as well as growth complementation of yeast deletion strains missing either RNA triphosphatase or guanylyltransferase required terminal sequences not present in the previously isolated cel-1 clone. By RNA interference analysis we show that cel-1 is required for embryogenesis. cel-1(RNAi) embryos formed cytoplasmic granules characteristic of a phenocluster of RNA processing genes and died early in development.

Original languageEnglish (US)
Pages (from-to)14168-14173
Number of pages6
JournalJournal of Biological Chemistry
Volume278
Issue number16
DOIs
StatePublished - Apr 18 2003

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mRNA guanylyltransferase
Caenorhabditis elegans
RNA Interference
Yeasts
RNA
Yeast
Cytoplasmic Granules
RNA Polymerase II
RNA Stability
RNA Caps
Embryonic Development
Proteins
Embryonic Structures
Clone Cells
Enzymes
Processing
Growth
Genes
Degradation

ASJC Scopus subject areas

  • Biochemistry

Cite this

mRNA capping enzyme requirement for Caenorhabditis elegans viability. / Srinivasan, Priya; Piano, Fabio; Shatkin, Aaron J.

In: Journal of Biological Chemistry, Vol. 278, No. 16, 18.04.2003, p. 14168-14173.

Research output: Contribution to journalArticle

Srinivasan, Priya ; Piano, Fabio ; Shatkin, Aaron J. / mRNA capping enzyme requirement for Caenorhabditis elegans viability. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 16. pp. 14168-14173.
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