Molten globular characteristics of the native state of apomyoglobin

Laura Lin, Rachel J. Pinker, Kirk Forde, George D. Rose, Neville R. Kallenbach

Research output: Contribution to journalArticle

Abstract

Apomyoglobin, myoglobin lacking the haem group, is a natural intermediate in biosynthesis of myoglobin, and has some structural features in common with the haem-containing native state. Unfolding or refolding studies of apomyoglobin have identified a molten globule intermediate at acid pH. We show here that both the native state of apomyoglobin and the molten globule intermediate have highly plastic structures. Substitution of single amino acids on the surface or in the interior of helices in the native protein produce dramatic changes in the helix content and tryptophan emission of apomyoglobin at neutral and acidic pH. The signals from the intermediate and native apomyoglobin correlate closely suggesting that apomyoglobin itself has a molten globule-like character, its structure representing a population of interconverting substates rather than a fixed conformation.

Original languageEnglish (US)
Pages (from-to)447-452
Number of pages6
JournalNature Structural Biology
Volume1
Issue number7
StatePublished - Jul 1994

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Molten materials
Myoglobin
Heme
Biosynthesis
Amino Acid Substitution
Tryptophan
Plastics
Conformations
apomyoglobin
Substitution reactions
Amino Acids
Acids
Population
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology

Cite this

Lin, L., Pinker, R. J., Forde, K., Rose, G. D., & Kallenbach, N. R. (1994). Molten globular characteristics of the native state of apomyoglobin. Nature Structural Biology, 1(7), 447-452.

Molten globular characteristics of the native state of apomyoglobin. / Lin, Laura; Pinker, Rachel J.; Forde, Kirk; Rose, George D.; Kallenbach, Neville R.

In: Nature Structural Biology, Vol. 1, No. 7, 07.1994, p. 447-452.

Research output: Contribution to journalArticle

Lin, L, Pinker, RJ, Forde, K, Rose, GD & Kallenbach, NR 1994, 'Molten globular characteristics of the native state of apomyoglobin', Nature Structural Biology, vol. 1, no. 7, pp. 447-452.
Lin L, Pinker RJ, Forde K, Rose GD, Kallenbach NR. Molten globular characteristics of the native state of apomyoglobin. Nature Structural Biology. 1994 Jul;1(7):447-452.
Lin, Laura ; Pinker, Rachel J. ; Forde, Kirk ; Rose, George D. ; Kallenbach, Neville R. / Molten globular characteristics of the native state of apomyoglobin. In: Nature Structural Biology. 1994 ; Vol. 1, No. 7. pp. 447-452.
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