Molecules of streptococcus gordonii that bind to porphyromonas gingivalis

R. J. Lamont, S. Gil, D. R. Demuth, Daniel Malamud, B. Rosan

Research output: Contribution to journalArticle

Abstract

Interbacterial binding is considered an important colonization mechanism for many of the organisms that inhabit dental plaque. Porphyromonas gingivalis, a periodontal pathogen, can adhere to species that comprise early plaque, such as Streptococcus gordonii. In this study, the molecules of S. gordonii G9B that mediate binding to P. gingivalis were investigated. Biotinylated surface molecules of S. gordonii were extracted and mixed with P. gingivalis cells. Interactive streptococcal components were identified by SDS-PAGE of the P. gingivalis cells followed by electroblotting, and visualization of the adsorbed streptococcal molecules with streptavidin-alkaline phosphatase. S. gordonii molecules of 45 kDa and a doublet of 62/60 kDa were observed to bind to P. gingivalis. Polyclonal antibodies raised to the 62/60 kDa proteins inhibited the binding interaction. These antibodies demonstrated an antigenic relationship between the 62/60 kDa molecules and the 45 kDa protein. Both molecules were also antigenically related to, and may be breakdown products of, a larger molecule of 170 kDa which is antigenically related to the P1 antigen of S. mutans. Cloning and expression in Enterococcus faecalis of the gene for the P1-like molecule from S. gordonii M5 resulted in a phenotype that expressed the 62/60 kDa and 45 kDa antigens and was capable of binding to P. gingivalis. These results suggest that a P1-1ike molecule in S. gordonii is involved in adherence to P. gingivalis. Processing of the P1-1ike molecule into smaller fragments of 62/60 kDa and 45 kDa may be required for binding activity.

Original languageEnglish (US)
Pages (from-to)867-872
Number of pages6
JournalMicrobiology
Volume140
Issue number4
StatePublished - 1994

Fingerprint

Streptococcus gordonii
Porphyromonas gingivalis
Antigens
Dental Plaque
Streptavidin
Antibodies
Enterococcus faecalis
Protein Binding
Alkaline Phosphatase
Organism Cloning
Polyacrylamide Gel Electrophoresis
Phenotype

Keywords

  • Interbacterial binding adhesin
  • P1
  • Porphyromonas gingivalis
  • Streptococcus gordonii

ASJC Scopus subject areas

  • Microbiology

Cite this

Lamont, R. J., Gil, S., Demuth, D. R., Malamud, D., & Rosan, B. (1994). Molecules of streptococcus gordonii that bind to porphyromonas gingivalis. Microbiology, 140(4), 867-872.

Molecules of streptococcus gordonii that bind to porphyromonas gingivalis. / Lamont, R. J.; Gil, S.; Demuth, D. R.; Malamud, Daniel; Rosan, B.

In: Microbiology, Vol. 140, No. 4, 1994, p. 867-872.

Research output: Contribution to journalArticle

Lamont, RJ, Gil, S, Demuth, DR, Malamud, D & Rosan, B 1994, 'Molecules of streptococcus gordonii that bind to porphyromonas gingivalis', Microbiology, vol. 140, no. 4, pp. 867-872.
Lamont, R. J. ; Gil, S. ; Demuth, D. R. ; Malamud, Daniel ; Rosan, B. / Molecules of streptococcus gordonii that bind to porphyromonas gingivalis. In: Microbiology. 1994 ; Vol. 140, No. 4. pp. 867-872.
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