Molecular profiling of oral microbiota in jawbone samples of bisphosphonate-related osteonecrosis of the jaw

X. Wei, S. Pushalkar, C. Estilo, C. Wong, A. Farooki, M. Fornier, G. Bohle, J. Huryn, Yihong Li, S. Doty, Deepak Saxena

Research output: Contribution to journalArticle

Abstract

Objective: Infection has been hypothesized as a contributing factor to bisphosphonate (BP)-related osteonecrosis of the jaw (BRONJ). The objective of this study was to determine the bacterial colonization of jawbone and identify the bacterial phylotypes associated with BRONJ. Materials and methods: Culture-independent 16S rRNA gene-based molecular techniques were used to determine and compare the total bacterial diversity in bone samples collected from 12 patients with cancer (six, BRONJ with history of BP; six, controls without BRONJ, no history of BP but have infection). Results: Denaturing gradient gel electrophoresis profile and Dice coefficient displayed a statistically significant clustering of profiles, indicating different bacterial population in BRONJ subjects and control. The top three genera ranked among the BRONJ group were Streptococcus (29%), Eubacterium (9%), and Pseudoramibacter (8%), while in the control group were Parvimonas (17%), Streptococcus (15%), and Fusobacterium (15%). H&E sections of BRONJ bone revealed layers of bacteria along the surfaces and often are packed into the scalloped edges of the bone. Conclusion: This study using limited sample size indicated that the jawbone associated with BRONJ was heavily colonized by specific oral bacteria and there were apparent differences between the microbiota of BRONJ and controls.

Original languageEnglish (US)
Pages (from-to)602-612
Number of pages11
JournalOral Diseases
Volume18
Issue number6
DOIs
StatePublished - Sep 2012

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Bisphosphonate-Associated Osteonecrosis of the Jaw
Osteonecrosis
Microbiota
Jaw
Diphosphonates
Streptococcus
Bone and Bones
Fusobacterium
Eubacterium
Bacteria
Denaturing Gradient Gel Electrophoresis
Infection
rRNA Genes
Sample Size
Cluster Analysis
Control Groups

Keywords

  • 16S rDNA
  • Bisphosphonate
  • Denaturing gradient gel electrophoresis
  • Microbial shift
  • Osteomyelitis
  • Osteonecrosis of the jaw

ASJC Scopus subject areas

  • Dentistry(all)
  • Otorhinolaryngology

Cite this

Molecular profiling of oral microbiota in jawbone samples of bisphosphonate-related osteonecrosis of the jaw. / Wei, X.; Pushalkar, S.; Estilo, C.; Wong, C.; Farooki, A.; Fornier, M.; Bohle, G.; Huryn, J.; Li, Yihong; Doty, S.; Saxena, Deepak.

In: Oral Diseases, Vol. 18, No. 6, 09.2012, p. 602-612.

Research output: Contribution to journalArticle

Wei, X, Pushalkar, S, Estilo, C, Wong, C, Farooki, A, Fornier, M, Bohle, G, Huryn, J, Li, Y, Doty, S & Saxena, D 2012, 'Molecular profiling of oral microbiota in jawbone samples of bisphosphonate-related osteonecrosis of the jaw', Oral Diseases, vol. 18, no. 6, pp. 602-612. https://doi.org/10.1111/j.1601-0825.2012.01916.x
Wei, X. ; Pushalkar, S. ; Estilo, C. ; Wong, C. ; Farooki, A. ; Fornier, M. ; Bohle, G. ; Huryn, J. ; Li, Yihong ; Doty, S. ; Saxena, Deepak. / Molecular profiling of oral microbiota in jawbone samples of bisphosphonate-related osteonecrosis of the jaw. In: Oral Diseases. 2012 ; Vol. 18, No. 6. pp. 602-612.
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