Molecular imaging using fluorescent lectins permits rapid endoscopic identification of dysplasia in Barrett's esophagus

Elizabeth L. Bird-Lieberman, André A. Neves, Pierre Lao-Sirieix, Maria O'Donovan, Marco Novelli, Laurence B. Lovat, William S. Eng, Lara Mahal, Kevin M. Brindle, Rebecca C. Fitzgerald

Research output: Contribution to journalArticle

Abstract

Barrett's esophagus is an example of a pre-invasive state, for which current endoscopic surveillance methods to detect dysplasia are time consuming and inadequate. The prognosis of cancer arising in Barrett's esophagus is improved by early detection at the stage of mucosal carcinoma or high-grade dysplasia. Molecular imaging methods could revolutionize the detection of dysplasia, provided they permit a wide field of view and highlight abnormalities in real time. We show here that cell-surface glycans are altered in the progression from Barrett's esophagus to adenocarcinoma and lead to specific changes in lectin binding patterns. We chose wheat germ agglutinin as a candidate lectin with clinical potential. The binding of wheat germ agglutinin to human tissue was determined to be specific, and we validated this specific binding by successful endoscopic visualization of high-grade dysplastic lesions, which were not detectable by conventional endoscopy, with a high signal-to-background ratio of over 5.

Original languageEnglish (US)
Pages (from-to)315-321
Number of pages7
JournalNature Medicine
Volume18
Issue number2
DOIs
StatePublished - Feb 2012

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Molecular imaging
Wheat Germ Agglutinins
Molecular Imaging
Barrett Esophagus
Lectins
Endoscopy
Polysaccharides
Visualization
Tissue
Adenocarcinoma
Carcinoma
Neoplasms

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Bird-Lieberman, E. L., Neves, A. A., Lao-Sirieix, P., O'Donovan, M., Novelli, M., Lovat, L. B., ... Fitzgerald, R. C. (2012). Molecular imaging using fluorescent lectins permits rapid endoscopic identification of dysplasia in Barrett's esophagus. Nature Medicine, 18(2), 315-321. https://doi.org/10.1038/nm.2616

Molecular imaging using fluorescent lectins permits rapid endoscopic identification of dysplasia in Barrett's esophagus. / Bird-Lieberman, Elizabeth L.; Neves, André A.; Lao-Sirieix, Pierre; O'Donovan, Maria; Novelli, Marco; Lovat, Laurence B.; Eng, William S.; Mahal, Lara; Brindle, Kevin M.; Fitzgerald, Rebecca C.

In: Nature Medicine, Vol. 18, No. 2, 02.2012, p. 315-321.

Research output: Contribution to journalArticle

Bird-Lieberman, EL, Neves, AA, Lao-Sirieix, P, O'Donovan, M, Novelli, M, Lovat, LB, Eng, WS, Mahal, L, Brindle, KM & Fitzgerald, RC 2012, 'Molecular imaging using fluorescent lectins permits rapid endoscopic identification of dysplasia in Barrett's esophagus', Nature Medicine, vol. 18, no. 2, pp. 315-321. https://doi.org/10.1038/nm.2616
Bird-Lieberman EL, Neves AA, Lao-Sirieix P, O'Donovan M, Novelli M, Lovat LB et al. Molecular imaging using fluorescent lectins permits rapid endoscopic identification of dysplasia in Barrett's esophagus. Nature Medicine. 2012 Feb;18(2):315-321. https://doi.org/10.1038/nm.2616
Bird-Lieberman, Elizabeth L. ; Neves, André A. ; Lao-Sirieix, Pierre ; O'Donovan, Maria ; Novelli, Marco ; Lovat, Laurence B. ; Eng, William S. ; Mahal, Lara ; Brindle, Kevin M. ; Fitzgerald, Rebecca C. / Molecular imaging using fluorescent lectins permits rapid endoscopic identification of dysplasia in Barrett's esophagus. In: Nature Medicine. 2012 ; Vol. 18, No. 2. pp. 315-321.
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