Molecular basis of the copulatory plug polymorphism in Caenorhabditis elegans

Michael F. Palopoli, Matthew Rockman, Aye TinMaung, Camden Ramsay, Stephen Curwen, Andrea Aduna, Jason Laurita, Leonid Kruglyak

Research output: Contribution to journalArticle

Abstract

Heritable variation is the raw material for evolutionary change, and understanding its genetic basis is one of the central problems in modern biology. We investigated the genetic basis of a classic phenotypic dimorphism in the nematode Caenorhabditis elegans. Males from many natural isolates deposit a copulatory plug after mating, whereas males from other natural isolates-including the standard wild-type strain (N2 Bristol) that is used in most research laboratories-do not deposit plugs. The copulatory plug is a gelatinous mass that covers the hermaphrodite vulva, and its deposition decreases the mating success of subsequent males. We show that the plugging polymorphism results from the insertion of a retrotransposon into an exon of a novel mucin-like gene, plg-1, whose product is a major structural component of the copulatory plug. The gene is expressed in a subset of secretory cells of the male somatic gonad, and its loss has no evident effects beyond the loss of male mate-guarding. Although C. elegans descends from an obligate-outcrossing, male?female ancestor, it occurs primarily as self-fertilizing hermaphrodites. The reduced selection on male-male competition associated with the origin of hermaphroditism may have permitted the global spread of a loss-of-function mutation with restricted pleiotropy.

Original languageEnglish (US)
Pages (from-to)1019-1022
Number of pages4
JournalNature
Volume454
Issue number7207
DOIs
StatePublished - Aug 21 2008

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Caenorhabditis elegans
Disorders of Sex Development
Retroelements
Vulva
Gonads
Mucins
Genes
Exons
Mutation
Research

ASJC Scopus subject areas

  • General

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Palopoli, M. F., Rockman, M., TinMaung, A., Ramsay, C., Curwen, S., Aduna, A., ... Kruglyak, L. (2008). Molecular basis of the copulatory plug polymorphism in Caenorhabditis elegans. Nature, 454(7207), 1019-1022. https://doi.org/10.1038/nature07171

Molecular basis of the copulatory plug polymorphism in Caenorhabditis elegans. / Palopoli, Michael F.; Rockman, Matthew; TinMaung, Aye; Ramsay, Camden; Curwen, Stephen; Aduna, Andrea; Laurita, Jason; Kruglyak, Leonid.

In: Nature, Vol. 454, No. 7207, 21.08.2008, p. 1019-1022.

Research output: Contribution to journalArticle

Palopoli, MF, Rockman, M, TinMaung, A, Ramsay, C, Curwen, S, Aduna, A, Laurita, J & Kruglyak, L 2008, 'Molecular basis of the copulatory plug polymorphism in Caenorhabditis elegans', Nature, vol. 454, no. 7207, pp. 1019-1022. https://doi.org/10.1038/nature07171
Palopoli MF, Rockman M, TinMaung A, Ramsay C, Curwen S, Aduna A et al. Molecular basis of the copulatory plug polymorphism in Caenorhabditis elegans. Nature. 2008 Aug 21;454(7207):1019-1022. https://doi.org/10.1038/nature07171
Palopoli, Michael F. ; Rockman, Matthew ; TinMaung, Aye ; Ramsay, Camden ; Curwen, Stephen ; Aduna, Andrea ; Laurita, Jason ; Kruglyak, Leonid. / Molecular basis of the copulatory plug polymorphism in Caenorhabditis elegans. In: Nature. 2008 ; Vol. 454, No. 7207. pp. 1019-1022.
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