Molecular architecture of SMC proteins and the yeast cohesin complex

Christian H. Haering, Jan Löwe, Andreas Hochwagen, Kim Nasmyth

Research output: Contribution to journalArticle

Abstract

Sister chromatids are held together by the multisubunit cohesin complex, which contains two SMC (Smc1 and Smc3) and two non-SMC (Scc1 and Scc3) proteins. The crystal structure of a bacterial SMC "hinge" region along with EM studies and biochemical experiments on yeast Smc1 and Smc3 proteins show that SMC protamers fold up individually into rod-shaped molecules. A 45 nm long intramolecular coiled coil separates the hinge region from the ATPase-containing "head" domain. Smc1 and Smc3 bind to each other via heterotypic interactions between their hinges to form a V-shaped heterodimer. The two heads of the V-shaped dimer are connected by different ends of the cleavable Scc1 subunit. Cohesin therefore forms a large proteinaceous loop within which sister chromatids might be entrapped after DNA replication.

Original languageEnglish (US)
Pages (from-to)773-788
Number of pages16
JournalMolecular Cell
Volume9
Issue number4
DOIs
StatePublished - 2002

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Chromatids
Head
Bacterial Structures
DNA Replication
Adenosine Triphosphatases
Proteins
Yeasts
cohesins

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Molecular architecture of SMC proteins and the yeast cohesin complex. / Haering, Christian H.; Löwe, Jan; Hochwagen, Andreas; Nasmyth, Kim.

In: Molecular Cell, Vol. 9, No. 4, 2002, p. 773-788.

Research output: Contribution to journalArticle

Haering, Christian H. ; Löwe, Jan ; Hochwagen, Andreas ; Nasmyth, Kim. / Molecular architecture of SMC proteins and the yeast cohesin complex. In: Molecular Cell. 2002 ; Vol. 9, No. 4. pp. 773-788.
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