Modulations of benzo[a]pyrene-induced DNA adduct, cyclin D1 and PCNA in oral tissue by 1,4-phenylenebis(methylene)selenocyanate

Kun Ming Chen, Peter G. Sacks, Thomas E. Spratt, Jyh Ming Lin, Telih Boyiri, Joel Schwartz, John P. Richie, Ana Calcagnotto, Arunangshu Das, James Bortner, Zonglin Zhao, Shantu Amin, Joseph Guttenplan, Karam El-Bayoumy

Research output: Contribution to journalArticle

Abstract

Tobacco smoking is an important cause of human oral squamous cell carcinoma (SCC). Tobacco smoke contains multiple carcinogens include polycyclic aromatic hydrocarbons typified by benzo[a]pyrene (B[a]P). Surgery is the conventional treatment approach for SCC, but it remains imperfect. However, chemoprevention is a plausible strategy and we had previously demonstrated that 1,4-phenylenebis(methylene)selenocyanate (p-XSC) significantly inhibited tongue tumors-induced by the synthetic 4-nitroquinoline-N-oxide (not present in tobacco smoke). In this study, we demonstrated that p-XSC is capable of inhibiting B[a]P-DNA adduct formation, cell proliferation, cyclin D1 expression in human oral cells in vitro. In addition, we showed that dietary p-XSC inhibits B[a]P-DNA adduct formation, cell proliferation and cyclin D1 protein expression in the mouse tongue in vivo. The results of this study are encouraging to further evaluate the chemopreventive efficacy of p-XSC initially against B[a]P-induced tongue tumors in mice and ultimately in the clinic.

Original languageEnglish (US)
Pages (from-to)151-155
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume383
Issue number1
DOIs
StatePublished - May 22 2009

Fingerprint

Tobacco
DNA Adducts
Benzo(a)pyrene
Cyclin D1
Proliferating Cell Nuclear Antigen
Tongue
Modulation
Cell proliferation
Tissue
Smoke
Tumors
Squamous Cell Carcinoma
Cell Proliferation
4-Nitroquinoline-1-oxide
Polycyclic Aromatic Hydrocarbons
Chemoprevention
Carcinogens
Surgery
Neoplasms
Smoking

Keywords

  • B[a]P
  • Chemoprevention
  • p-XSC
  • Selenium

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Modulations of benzo[a]pyrene-induced DNA adduct, cyclin D1 and PCNA in oral tissue by 1,4-phenylenebis(methylene)selenocyanate. / Chen, Kun Ming; Sacks, Peter G.; Spratt, Thomas E.; Lin, Jyh Ming; Boyiri, Telih; Schwartz, Joel; Richie, John P.; Calcagnotto, Ana; Das, Arunangshu; Bortner, James; Zhao, Zonglin; Amin, Shantu; Guttenplan, Joseph; El-Bayoumy, Karam.

In: Biochemical and Biophysical Research Communications, Vol. 383, No. 1, 22.05.2009, p. 151-155.

Research output: Contribution to journalArticle

Chen, KM, Sacks, PG, Spratt, TE, Lin, JM, Boyiri, T, Schwartz, J, Richie, JP, Calcagnotto, A, Das, A, Bortner, J, Zhao, Z, Amin, S, Guttenplan, J & El-Bayoumy, K 2009, 'Modulations of benzo[a]pyrene-induced DNA adduct, cyclin D1 and PCNA in oral tissue by 1,4-phenylenebis(methylene)selenocyanate', Biochemical and Biophysical Research Communications, vol. 383, no. 1, pp. 151-155. https://doi.org/10.1016/j.bbrc.2009.03.145
Chen, Kun Ming ; Sacks, Peter G. ; Spratt, Thomas E. ; Lin, Jyh Ming ; Boyiri, Telih ; Schwartz, Joel ; Richie, John P. ; Calcagnotto, Ana ; Das, Arunangshu ; Bortner, James ; Zhao, Zonglin ; Amin, Shantu ; Guttenplan, Joseph ; El-Bayoumy, Karam. / Modulations of benzo[a]pyrene-induced DNA adduct, cyclin D1 and PCNA in oral tissue by 1,4-phenylenebis(methylene)selenocyanate. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 383, No. 1. pp. 151-155.
@article{0f0bd0c9f81b4543b629d8f4eb7745c5,
title = "Modulations of benzo[a]pyrene-induced DNA adduct, cyclin D1 and PCNA in oral tissue by 1,4-phenylenebis(methylene)selenocyanate",
abstract = "Tobacco smoking is an important cause of human oral squamous cell carcinoma (SCC). Tobacco smoke contains multiple carcinogens include polycyclic aromatic hydrocarbons typified by benzo[a]pyrene (B[a]P). Surgery is the conventional treatment approach for SCC, but it remains imperfect. However, chemoprevention is a plausible strategy and we had previously demonstrated that 1,4-phenylenebis(methylene)selenocyanate (p-XSC) significantly inhibited tongue tumors-induced by the synthetic 4-nitroquinoline-N-oxide (not present in tobacco smoke). In this study, we demonstrated that p-XSC is capable of inhibiting B[a]P-DNA adduct formation, cell proliferation, cyclin D1 expression in human oral cells in vitro. In addition, we showed that dietary p-XSC inhibits B[a]P-DNA adduct formation, cell proliferation and cyclin D1 protein expression in the mouse tongue in vivo. The results of this study are encouraging to further evaluate the chemopreventive efficacy of p-XSC initially against B[a]P-induced tongue tumors in mice and ultimately in the clinic.",
keywords = "B[a]P, Chemoprevention, p-XSC, Selenium",
author = "Chen, {Kun Ming} and Sacks, {Peter G.} and Spratt, {Thomas E.} and Lin, {Jyh Ming} and Telih Boyiri and Joel Schwartz and Richie, {John P.} and Ana Calcagnotto and Arunangshu Das and James Bortner and Zonglin Zhao and Shantu Amin and Joseph Guttenplan and Karam El-Bayoumy",
year = "2009",
month = "5",
day = "22",
doi = "10.1016/j.bbrc.2009.03.145",
language = "English (US)",
volume = "383",
pages = "151--155",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Modulations of benzo[a]pyrene-induced DNA adduct, cyclin D1 and PCNA in oral tissue by 1,4-phenylenebis(methylene)selenocyanate

AU - Chen, Kun Ming

AU - Sacks, Peter G.

AU - Spratt, Thomas E.

AU - Lin, Jyh Ming

AU - Boyiri, Telih

AU - Schwartz, Joel

AU - Richie, John P.

AU - Calcagnotto, Ana

AU - Das, Arunangshu

AU - Bortner, James

AU - Zhao, Zonglin

AU - Amin, Shantu

AU - Guttenplan, Joseph

AU - El-Bayoumy, Karam

PY - 2009/5/22

Y1 - 2009/5/22

N2 - Tobacco smoking is an important cause of human oral squamous cell carcinoma (SCC). Tobacco smoke contains multiple carcinogens include polycyclic aromatic hydrocarbons typified by benzo[a]pyrene (B[a]P). Surgery is the conventional treatment approach for SCC, but it remains imperfect. However, chemoprevention is a plausible strategy and we had previously demonstrated that 1,4-phenylenebis(methylene)selenocyanate (p-XSC) significantly inhibited tongue tumors-induced by the synthetic 4-nitroquinoline-N-oxide (not present in tobacco smoke). In this study, we demonstrated that p-XSC is capable of inhibiting B[a]P-DNA adduct formation, cell proliferation, cyclin D1 expression in human oral cells in vitro. In addition, we showed that dietary p-XSC inhibits B[a]P-DNA adduct formation, cell proliferation and cyclin D1 protein expression in the mouse tongue in vivo. The results of this study are encouraging to further evaluate the chemopreventive efficacy of p-XSC initially against B[a]P-induced tongue tumors in mice and ultimately in the clinic.

AB - Tobacco smoking is an important cause of human oral squamous cell carcinoma (SCC). Tobacco smoke contains multiple carcinogens include polycyclic aromatic hydrocarbons typified by benzo[a]pyrene (B[a]P). Surgery is the conventional treatment approach for SCC, but it remains imperfect. However, chemoprevention is a plausible strategy and we had previously demonstrated that 1,4-phenylenebis(methylene)selenocyanate (p-XSC) significantly inhibited tongue tumors-induced by the synthetic 4-nitroquinoline-N-oxide (not present in tobacco smoke). In this study, we demonstrated that p-XSC is capable of inhibiting B[a]P-DNA adduct formation, cell proliferation, cyclin D1 expression in human oral cells in vitro. In addition, we showed that dietary p-XSC inhibits B[a]P-DNA adduct formation, cell proliferation and cyclin D1 protein expression in the mouse tongue in vivo. The results of this study are encouraging to further evaluate the chemopreventive efficacy of p-XSC initially against B[a]P-induced tongue tumors in mice and ultimately in the clinic.

KW - B[a]P

KW - Chemoprevention

KW - p-XSC

KW - Selenium

UR - http://www.scopus.com/inward/record.url?scp=64949118574&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=64949118574&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2009.03.145

DO - 10.1016/j.bbrc.2009.03.145

M3 - Article

C2 - 19344691

AN - SCOPUS:64949118574

VL - 383

SP - 151

EP - 155

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -