Modulation of the frequency of glucose-dependent bursts of electrical activity by HCO3/CO2 in rodent pancreatic B-cells

experimental and theoretical results

P. B. Carroll, A. Sherman, R. Ferrer, A. C. Boschero, J. Rinzel, I. Atwater

Research output: Contribution to journalArticle

Abstract

The burst pattern of electrical activity recorded from pancreatic B-cells in response to 11 m M glucose shows a large islet to islet variability. The relationship between burst frequency and glucose sensing (the threshold for electrical activity and the graded increase in electrical response to glucose, i.e. active phase %) has not been investigated within the same islet. In this work, we show that low HCO3 (5 m M) Hepes buffered solutions reversibly reduce the frequency of bursts compared to control (25 m M) HCO3 buffered solutions in the same islet. There was no change in the threshold or active phase (%). Using the mathematical model of Sherman et al. 1988, we explored mechanisms for a change in frequency independent of a change in active phase (%). Increased exchangeable calcium pool size and increased cell to cell coupling were the two theoretical treatments which could reproduce the experimental data. We conclude that burst frequency can be modulated independent of the active phase and that alteration of a calcium pool size best fits the experimental data.

Original languageEnglish (US)
Pages (from-to)71-77
Number of pages7
JournalEuropean Biophysics Journal
Volume18
Issue number2
DOIs
StatePublished - Mar 1990

Fingerprint

Insulin-Secreting Cells
Rodentia
Glucose
Calcium
Cell Size
Theoretical Models

Keywords

  • Burst frequency modulation
  • HCO/CO
  • Mathematical model
  • Pancreatic B-cell

ASJC Scopus subject areas

  • Biophysics

Cite this

Modulation of the frequency of glucose-dependent bursts of electrical activity by HCO3/CO2 in rodent pancreatic B-cells : experimental and theoretical results. / Carroll, P. B.; Sherman, A.; Ferrer, R.; Boschero, A. C.; Rinzel, J.; Atwater, I.

In: European Biophysics Journal, Vol. 18, No. 2, 03.1990, p. 71-77.

Research output: Contribution to journalArticle

@article{ac1ebfd0667445849fae140dfef69fe7,
title = "Modulation of the frequency of glucose-dependent bursts of electrical activity by HCO3/CO2 in rodent pancreatic B-cells: experimental and theoretical results",
abstract = "The burst pattern of electrical activity recorded from pancreatic B-cells in response to 11 m M glucose shows a large islet to islet variability. The relationship between burst frequency and glucose sensing (the threshold for electrical activity and the graded increase in electrical response to glucose, i.e. active phase {\%}) has not been investigated within the same islet. In this work, we show that low HCO3 (5 m M) Hepes buffered solutions reversibly reduce the frequency of bursts compared to control (25 m M) HCO3 buffered solutions in the same islet. There was no change in the threshold or active phase ({\%}). Using the mathematical model of Sherman et al. 1988, we explored mechanisms for a change in frequency independent of a change in active phase ({\%}). Increased exchangeable calcium pool size and increased cell to cell coupling were the two theoretical treatments which could reproduce the experimental data. We conclude that burst frequency can be modulated independent of the active phase and that alteration of a calcium pool size best fits the experimental data.",
keywords = "Burst frequency modulation, HCO/CO, Mathematical model, Pancreatic B-cell",
author = "Carroll, {P. B.} and A. Sherman and R. Ferrer and Boschero, {A. C.} and J. Rinzel and I. Atwater",
year = "1990",
month = "3",
doi = "10.1007/BF00183265",
language = "English (US)",
volume = "18",
pages = "71--77",
journal = "European Biophysics Journal",
issn = "0175-7571",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Modulation of the frequency of glucose-dependent bursts of electrical activity by HCO3/CO2 in rodent pancreatic B-cells

T2 - experimental and theoretical results

AU - Carroll, P. B.

AU - Sherman, A.

AU - Ferrer, R.

AU - Boschero, A. C.

AU - Rinzel, J.

AU - Atwater, I.

PY - 1990/3

Y1 - 1990/3

N2 - The burst pattern of electrical activity recorded from pancreatic B-cells in response to 11 m M glucose shows a large islet to islet variability. The relationship between burst frequency and glucose sensing (the threshold for electrical activity and the graded increase in electrical response to glucose, i.e. active phase %) has not been investigated within the same islet. In this work, we show that low HCO3 (5 m M) Hepes buffered solutions reversibly reduce the frequency of bursts compared to control (25 m M) HCO3 buffered solutions in the same islet. There was no change in the threshold or active phase (%). Using the mathematical model of Sherman et al. 1988, we explored mechanisms for a change in frequency independent of a change in active phase (%). Increased exchangeable calcium pool size and increased cell to cell coupling were the two theoretical treatments which could reproduce the experimental data. We conclude that burst frequency can be modulated independent of the active phase and that alteration of a calcium pool size best fits the experimental data.

AB - The burst pattern of electrical activity recorded from pancreatic B-cells in response to 11 m M glucose shows a large islet to islet variability. The relationship between burst frequency and glucose sensing (the threshold for electrical activity and the graded increase in electrical response to glucose, i.e. active phase %) has not been investigated within the same islet. In this work, we show that low HCO3 (5 m M) Hepes buffered solutions reversibly reduce the frequency of bursts compared to control (25 m M) HCO3 buffered solutions in the same islet. There was no change in the threshold or active phase (%). Using the mathematical model of Sherman et al. 1988, we explored mechanisms for a change in frequency independent of a change in active phase (%). Increased exchangeable calcium pool size and increased cell to cell coupling were the two theoretical treatments which could reproduce the experimental data. We conclude that burst frequency can be modulated independent of the active phase and that alteration of a calcium pool size best fits the experimental data.

KW - Burst frequency modulation

KW - HCO/CO

KW - Mathematical model

KW - Pancreatic B-cell

UR - http://www.scopus.com/inward/record.url?scp=0025313848&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025313848&partnerID=8YFLogxK

U2 - 10.1007/BF00183265

DO - 10.1007/BF00183265

M3 - Article

VL - 18

SP - 71

EP - 77

JO - European Biophysics Journal

JF - European Biophysics Journal

SN - 0175-7571

IS - 2

ER -