Modulation of excitability in Aplysia tail sensory neurons by tyrosine kinases

Angela L. Purcell, Thomas Carew

Research output: Contribution to journalArticle

Abstract

Tyrosine kinases have recently been shown to modulate synaptic plasticity and ion channel function. We show here that tyrosine kinases can also modulate both the baseline excitability state of Aplysia tail sensory neurons (SNs) as well as the excitability induced by the neuromodulator serotonin (5HT). First, we examined the effects of increasing and decreasing tyrosine kinase activity in the SNs. We found that tyrosine kinase inhibitors decrease baseline SN excitability in addition to attenuating the increase in excitability induced by 5HT. Conversely, functionally increasing cellular tyrosine kinase activity in the SNs by either inhibiting opposing tyrosine phosphatase activity or by direct injection of an active tyrosine kinase (Src) induces increases in SN excitability in the absence of 5HT. Second, we examined the interaction between protein kinase A (PKA), which is known to mediate 5HT-induced excitability changes in the SNs, and tyrosine kinases, in the enhancement of SN excitability. We found that the tyrosine kinases function downstream of PKA activation since tyrosine kinase inhibitors reduce excitability induced by activators of PKA. Finally, we examined the role of tyrosine kinases in other forms of 5HT-induced plasticity in the SNs. We found that while tyrosine kinase inhibitors attenuate excitability produced by 5HT, they have no effect on short-term facilitation (STF) of the SN-motor neuron (MN) synapse induced by 5HT. Thus tyrosine kinases modulate different forms of SN plasticity independently. Such differential modulation would have important consequences for activity-dependent plasticity in a variety of neural circuits.

Original languageEnglish (US)
Pages (from-to)2398-2411
Number of pages14
JournalJournal of Neurophysiology
Volume85
Issue number6
StatePublished - 2001

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Aplysia
Sensory Receptor Cells
Protein-Tyrosine Kinases
Tail
Cyclic AMP-Dependent Protein Kinases
Neuronal Plasticity
src-Family Kinases
Motor Neurons
Ion Channels
Phosphoric Monoester Hydrolases
Synapses
Neurotransmitter Agents
Tyrosine
Serotonin

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

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Modulation of excitability in Aplysia tail sensory neurons by tyrosine kinases. / Purcell, Angela L.; Carew, Thomas.

In: Journal of Neurophysiology, Vol. 85, No. 6, 2001, p. 2398-2411.

Research output: Contribution to journalArticle

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