Modulation of β-receptors as adult and neonatal cardiac myocytes progress into culture

Evy Lundgren, Louis Terracio, Donald O. Allen, Thomas K. Borg

Research output: Contribution to journalArticle

Abstract

Modulation of β-adrenergic receptors and their ability to respond to β-receptor stimulation was studied in cultures of adult and neonatal rat cardiac myocytes. The radioligand iodocyanopindolol (125I-CYP) was used to identify β-adrenoceptors on the intact cells.125I-CYP was found to bind to the receptors in a stereospecific and saturable manner. Freshly isolated neonatal and adult myocytes both had a receptor density of approximately 50 fmol/mg protein. The number of β-receptors per milligram protein was similar during a 10-d culture period for adult myocytes but increased after a 5-d culture period for neonatal myocytes. Both cell types responded to β-receptor stimulation with isoproterenol by a twofold increase in the concentration of cAMP and this response increased with time in culture. The number of receptors as well as the response to isoproterenol was similar for neonatal myocytes cultured on laminin, collagen type I, or on uncoated culture dishes. From these data we conclude that cultured cardiac myocytes maintain functional β-receptors as they progress into culture, and the expression of β-receptors is not influenced by culture substrates.

Original languageEnglish (US)
Pages (from-to)28-34
Number of pages7
JournalIn vitro cellular & developmental biology : journal of the Tissue Culture Association
Volume24
Issue number1
DOIs
StatePublished - Jan 1988

Fingerprint

Isoproterenol
Cardiac Myocytes
Adrenergic Receptors
Muscle Cells
Iodocyanopindolol
Modulation
Laminin
Collagen Type I
Rats
Proteins
Substrates

Keywords

  • β-receptors
  • cAMP
  • cell-culture
  • ECM-components
  • myocytes

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Clinical Biochemistry

Cite this

Modulation of β-receptors as adult and neonatal cardiac myocytes progress into culture. / Lundgren, Evy; Terracio, Louis; Allen, Donald O.; Borg, Thomas K.

In: In vitro cellular & developmental biology : journal of the Tissue Culture Association, Vol. 24, No. 1, 01.1988, p. 28-34.

Research output: Contribution to journalArticle

@article{273038868af249469dd9e0c3f281e770,
title = "Modulation of β-receptors as adult and neonatal cardiac myocytes progress into culture",
abstract = "Modulation of β-adrenergic receptors and their ability to respond to β-receptor stimulation was studied in cultures of adult and neonatal rat cardiac myocytes. The radioligand iodocyanopindolol (125I-CYP) was used to identify β-adrenoceptors on the intact cells.125I-CYP was found to bind to the receptors in a stereospecific and saturable manner. Freshly isolated neonatal and adult myocytes both had a receptor density of approximately 50 fmol/mg protein. The number of β-receptors per milligram protein was similar during a 10-d culture period for adult myocytes but increased after a 5-d culture period for neonatal myocytes. Both cell types responded to β-receptor stimulation with isoproterenol by a twofold increase in the concentration of cAMP and this response increased with time in culture. The number of receptors as well as the response to isoproterenol was similar for neonatal myocytes cultured on laminin, collagen type I, or on uncoated culture dishes. From these data we conclude that cultured cardiac myocytes maintain functional β-receptors as they progress into culture, and the expression of β-receptors is not influenced by culture substrates.",
keywords = "β-receptors, cAMP, cell-culture, ECM-components, myocytes",
author = "Evy Lundgren and Louis Terracio and Allen, {Donald O.} and Borg, {Thomas K.}",
year = "1988",
month = "1",
doi = "10.1007/BF02623812",
language = "English (US)",
volume = "24",
pages = "28--34",
journal = "In Vitro Cellular & Developmental Biology",
issn = "0883-8364",
publisher = "Society for In Vitro Biology",
number = "1",

}

TY - JOUR

T1 - Modulation of β-receptors as adult and neonatal cardiac myocytes progress into culture

AU - Lundgren, Evy

AU - Terracio, Louis

AU - Allen, Donald O.

AU - Borg, Thomas K.

PY - 1988/1

Y1 - 1988/1

N2 - Modulation of β-adrenergic receptors and their ability to respond to β-receptor stimulation was studied in cultures of adult and neonatal rat cardiac myocytes. The radioligand iodocyanopindolol (125I-CYP) was used to identify β-adrenoceptors on the intact cells.125I-CYP was found to bind to the receptors in a stereospecific and saturable manner. Freshly isolated neonatal and adult myocytes both had a receptor density of approximately 50 fmol/mg protein. The number of β-receptors per milligram protein was similar during a 10-d culture period for adult myocytes but increased after a 5-d culture period for neonatal myocytes. Both cell types responded to β-receptor stimulation with isoproterenol by a twofold increase in the concentration of cAMP and this response increased with time in culture. The number of receptors as well as the response to isoproterenol was similar for neonatal myocytes cultured on laminin, collagen type I, or on uncoated culture dishes. From these data we conclude that cultured cardiac myocytes maintain functional β-receptors as they progress into culture, and the expression of β-receptors is not influenced by culture substrates.

AB - Modulation of β-adrenergic receptors and their ability to respond to β-receptor stimulation was studied in cultures of adult and neonatal rat cardiac myocytes. The radioligand iodocyanopindolol (125I-CYP) was used to identify β-adrenoceptors on the intact cells.125I-CYP was found to bind to the receptors in a stereospecific and saturable manner. Freshly isolated neonatal and adult myocytes both had a receptor density of approximately 50 fmol/mg protein. The number of β-receptors per milligram protein was similar during a 10-d culture period for adult myocytes but increased after a 5-d culture period for neonatal myocytes. Both cell types responded to β-receptor stimulation with isoproterenol by a twofold increase in the concentration of cAMP and this response increased with time in culture. The number of receptors as well as the response to isoproterenol was similar for neonatal myocytes cultured on laminin, collagen type I, or on uncoated culture dishes. From these data we conclude that cultured cardiac myocytes maintain functional β-receptors as they progress into culture, and the expression of β-receptors is not influenced by culture substrates.

KW - β-receptors

KW - cAMP

KW - cell-culture

KW - ECM-components

KW - myocytes

UR - http://www.scopus.com/inward/record.url?scp=0023852192&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023852192&partnerID=8YFLogxK

U2 - 10.1007/BF02623812

DO - 10.1007/BF02623812

M3 - Article

VL - 24

SP - 28

EP - 34

JO - In Vitro Cellular & Developmental Biology

JF - In Vitro Cellular & Developmental Biology

SN - 0883-8364

IS - 1

ER -