Modulating Self-Assembly of DNA Crystals with Rationally Designed Agents

Jiemin Zhao, Yue Zhao, Zhe Li, Yong Wang, Ruojie Sha, Nadrian Seeman, Chengde Mao

Research output: Contribution to journalArticle

Abstract

This manuscript reports a strategy for controlling the crystallization kinetics and improving the quality of engineered self-assembled 3D DNA crystals. Growing large, high-quality biomacromolecule crystals is critically important for determining the 3D structures of biomacromolecules. It often presents a great challenge to structural biologists. Herein, we introduce a rationally designed agent to modulate the crystallization process. Under such conditions, fewer, but larger, crystals that yield diffraction patterns of modestly higher resolution are produced compared with the crystals from conditions without the modulating agent. We attribute the improvement to a smaller number of nuclei and slow growth rate of crystallization. This strategy is expected to be generally applicable for crystallization of other biomacromolecules.

Original languageEnglish (US)
JournalAngewandte Chemie - International Edition
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Self assembly
DNA
Crystallization
Crystals
Crystallization kinetics
Diffraction patterns

Keywords

  • DNA
  • DNA crystals
  • DNA nanostructures
  • DNA nanotechnology
  • self-assembly

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Modulating Self-Assembly of DNA Crystals with Rationally Designed Agents. / Zhao, Jiemin; Zhao, Yue; Li, Zhe; Wang, Yong; Sha, Ruojie; Seeman, Nadrian; Mao, Chengde.

In: Angewandte Chemie - International Edition, 01.01.2018.

Research output: Contribution to journalArticle

Zhao, Jiemin ; Zhao, Yue ; Li, Zhe ; Wang, Yong ; Sha, Ruojie ; Seeman, Nadrian ; Mao, Chengde. / Modulating Self-Assembly of DNA Crystals with Rationally Designed Agents. In: Angewandte Chemie - International Edition. 2018.
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