MiRNAs are Essential for the Survival and Maturation of Cortical Interneurons

Sebnem N. Tuncdemir, Gordon Fishell, Renata Batista-Brito

    Research output: Contribution to journalArticle

    Abstract

    Complex and precisely orchestrated genetic programs contribute to the generation, migration, and maturation of cortical GABAergic interneurons (cIN). Yet, little is known about the signals that mediate the rapid alterations in gene expression that are required for cINs to transit through a series of developmental steps leading to their mature properties in the cortex. Here, we investigated the function of post-transcriptional regulation of gene expression by microRNAs on the development of cIN precursors. We find that conditional removal of the RNAseIII enzyme Dicer reduces the number of cINs in the adult mouse. Dicer is further necessary for the morphological and molecular maturation of cINs. Loss of mature miRNAs affects cINs development by impairing migration and differentiation of this cell type, while leaving proliferation of progenitors unperturbed. These developmental defects closely matched the abnormal expression of molecules involved in apoptosis and neuronal specification. In addition, we identified several miRNAs that are selectively upregulated in the postmitotic cINs, consistent with a role of miRNAs in the post-transcriptional control of the differentiation and apoptotic programs essential for cIN maturation. Thus, our results indicate that cIN progenitors require Dicer-dependent mechanisms to fine-tune the migration and maturation of cINs.

    Original languageEnglish (US)
    Pages (from-to)1842-1857
    Number of pages16
    JournalCerebral Cortex
    Volume25
    Issue number7
    DOIs
    StatePublished - Jan 1 2015

    Fingerprint

    Interneurons
    MicroRNAs
    Ribonuclease III
    Gene Expression Regulation
    Cell Movement
    Cell Differentiation
    Apoptosis
    Gene Expression

    Keywords

    • cortical interneurons
    • dicer
    • microRNA
    • post-transcriptional regulation

    ASJC Scopus subject areas

    • Cognitive Neuroscience
    • Cellular and Molecular Neuroscience

    Cite this

    MiRNAs are Essential for the Survival and Maturation of Cortical Interneurons. / Tuncdemir, Sebnem N.; Fishell, Gordon; Batista-Brito, Renata.

    In: Cerebral Cortex, Vol. 25, No. 7, 01.01.2015, p. 1842-1857.

    Research output: Contribution to journalArticle

    Tuncdemir, SN, Fishell, G & Batista-Brito, R 2015, 'MiRNAs are Essential for the Survival and Maturation of Cortical Interneurons', Cerebral Cortex, vol. 25, no. 7, pp. 1842-1857. https://doi.org/10.1093/cercor/bht426
    Tuncdemir, Sebnem N. ; Fishell, Gordon ; Batista-Brito, Renata. / MiRNAs are Essential for the Survival and Maturation of Cortical Interneurons. In: Cerebral Cortex. 2015 ; Vol. 25, No. 7. pp. 1842-1857.
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