MiR-Synth: A computational resource for the design of multi-site multi-target synthetic miRNAs

Alessandro Laganà, Mario Acunzo, Giulia Romano, Alfredo Pulvirenti, Dario Veneziano, Luciano Cascione, Rosalba Giugno, Pierluigi Gasparini, Dennis Shasha, Alfredo Ferro, Carlo Maria Croce

Research output: Contribution to journalArticle

Abstract

RNAi is a powerful tool for the regulation of gene expression. It is widely and successfully employed in functional studies and is now emerging as a promising therapeutic approach. Several RNAi-based clinical trials suggest encouraging results in the treatment of a variety of diseases, including cancer. Here we present miR-Synth, a computational resource for the design of synthetic microRNAs able to target multiple genes in multiple sites. The proposed strategy constitutes a valid alternative to the use of siRNA, allowing the employment of a fewer number of molecules for the inhibition of multiple targets. This may represent a great advantage in designing therapies for diseases caused by crucial cellular pathways altered by multiple dysregulated genes. The system has been successfully validated on two of the most prominent genes associated to lung cancer, c-MET and Epidermal Growth Factor Receptor (EGFR). (See http://microrna.osumc.edu/mir-synth).

Original languageEnglish (US)
Pages (from-to)5416-5425
Number of pages10
JournalNucleic Acids Research
Volume42
Issue number9
DOIs
StatePublished - 2014

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MicroRNAs
RNA Interference
Genes
Gene Expression Regulation
Epidermal Growth Factor Receptor
Small Interfering RNA
Lung Neoplasms
Clinical Trials
Therapeutics
Neoplasms

ASJC Scopus subject areas

  • Genetics

Cite this

Laganà, A., Acunzo, M., Romano, G., Pulvirenti, A., Veneziano, D., Cascione, L., ... Croce, C. M. (2014). MiR-Synth: A computational resource for the design of multi-site multi-target synthetic miRNAs. Nucleic Acids Research, 42(9), 5416-5425. https://doi.org/10.1093/nar/gku202

MiR-Synth : A computational resource for the design of multi-site multi-target synthetic miRNAs. / Laganà, Alessandro; Acunzo, Mario; Romano, Giulia; Pulvirenti, Alfredo; Veneziano, Dario; Cascione, Luciano; Giugno, Rosalba; Gasparini, Pierluigi; Shasha, Dennis; Ferro, Alfredo; Croce, Carlo Maria.

In: Nucleic Acids Research, Vol. 42, No. 9, 2014, p. 5416-5425.

Research output: Contribution to journalArticle

Laganà, A, Acunzo, M, Romano, G, Pulvirenti, A, Veneziano, D, Cascione, L, Giugno, R, Gasparini, P, Shasha, D, Ferro, A & Croce, CM 2014, 'MiR-Synth: A computational resource for the design of multi-site multi-target synthetic miRNAs', Nucleic Acids Research, vol. 42, no. 9, pp. 5416-5425. https://doi.org/10.1093/nar/gku202
Laganà A, Acunzo M, Romano G, Pulvirenti A, Veneziano D, Cascione L et al. MiR-Synth: A computational resource for the design of multi-site multi-target synthetic miRNAs. Nucleic Acids Research. 2014;42(9):5416-5425. https://doi.org/10.1093/nar/gku202
Laganà, Alessandro ; Acunzo, Mario ; Romano, Giulia ; Pulvirenti, Alfredo ; Veneziano, Dario ; Cascione, Luciano ; Giugno, Rosalba ; Gasparini, Pierluigi ; Shasha, Dennis ; Ferro, Alfredo ; Croce, Carlo Maria. / MiR-Synth : A computational resource for the design of multi-site multi-target synthetic miRNAs. In: Nucleic Acids Research. 2014 ; Vol. 42, No. 9. pp. 5416-5425.
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